Abstract
The influence of chlorpromazine (CP) on the incorporation of 3H-uridine into RNA and 3H-histidine into proteins of the optic tectum was investigated in Brachydanio rerio (Teleosts) by means of autoradiography. In addition, the effect of different concentrations of the drug (1–128 mg CP/l bathing medium) on the behaviour of the fish was examined. The LD50 was 2 mg CP/l. 1.5 mg CP/l, a sublethal dose which induces behaviour changes, slightly inhibited RNA synthesis. On the other hand, the same dose has a marked effect on protein metabolism; both total incorporation of 3H-histidine into the cell bodies as well as protein breakdown in the nerve fibres appeared to be inhibited as indicated by a decreased labelling of the pericarya after short periods and an increased accumulation of label in the nerve fibres after long periods of incorporation. Moreover the 3H-histidine labelling in the optic tectum was less affected by exogenous protease in CP-treated animals than in controls. The possible sites of action of the drug are discussed.
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Die Untersuchungen wurden durch Herrn Prof. Dr. H. Rahmann gewährte Sachmittel der Deutschen Forschungsgemeinschaft sowie das Land Nordrhein-Westfalen unterstützt.
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Rösner, H. Untersuchungen zur Wirkung von Chlorpromazin im ZNS von Teleosteern. Psychopharmacologia 23, 125–135 (1972). https://doi.org/10.1007/BF00401187
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DOI: https://doi.org/10.1007/BF00401187