Summary
The fractional plasma escape rates of glycated and non-glycated albumin have earlier been measured in groups of Type 1 (insulin-dependent) diabetic patients and control subjects. The escape of non-glycated albumin was similar in control subjects and normoalbuminuric patients, but elevated in patients with micro or macroalbuminuria. In all groups the escape rate of glycated albumin was lower than that of non-glycated albumin. Glycation increases the anionic charge of albumin. To assay for charge-dependent alterations of transport a selectivity index (non-glycated albumin/glycated albumin transport ratio) was determined from the disappearance data. The index was high in control subjects (1.021±0.0057 (SEM)). This reflects a mean difference between the two escape rates of 2.1% per hour (for comparison the mean of the fractional escape rate of non-glycated albumin of the normal control subjects was 4.7% per hour). The index was numerically even higher in normoalbuminuric patients (1.031±0.0047 (SEM)), but reached significantly lower levels in patients with microalbuminuria (1.013±0.0030 (SEM), p<0.02). Patients with clinical nephropathy had very low levels indicating loss of selectivity (1.002±0.0068 (SEM), p<0.001). This pattern accords well with measurements of renal clearance selectivity indices, suggesting a general, progressive deterioration of anionic perivascular barrier components in diabetic microangiopathy. The structural target for these changes is likely to be the glycosaminoglycans of the glomerular basal membrane and the interstitial matrix.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Parving H-H, Rasmussen SM (1973) Transcapillary escape rate of albumin in short- and long-term juvenile diabetics. Scand J Clin Lab Invest 32: 81–87
Feldt-Rasmussen B (1986) Increased transcapillary escape rate of albumin in type 1 (insulin-dependent) diabetic patients with microalbuminuria. Diabetologia 29: 282–286
Bent-Hansen L, Feldt-Rasmussen B, Kverneland A, Deckert T (1987) Transcapillary escape rate and relative metabolic clearance of glycated and non-glycated albumin in type 1 (insulin-dependent) diabetes mellitus. Diabetologia 30: 2–4
Viberti GC, Mackintosh D, Keen H (1983) Determinants of the penetration of proteins through the glomerular barrier in insulin-dependent diabetes mellitus. Diabetes 32 [Suppl 2]: 92–95
Kverneland A, Feldt-Rasmussen B, Vidal P et al. (1986) Evidence of changes in renal charge selectivity in patients with type 1 (insulin-dependent) diabetes mellitus. Diabetologia 29: 634–639
Deckert T, Kofoed-Enevoldsen A, Vidal P, Nørgaard K, Andreassen HB, Feldt-Rasmussen B (1993) Size and charge selectivity of glomerular filtration in type 1 (insulin-dependent) diabetic patients with and without albuminuria. Diabetologia 36: 244–251
Vidal P, Deckert T, Hansen B, Welinder WS (1989) High performance liquid chromatofocusing and column affinity chromatography of in vitro 14C-glycated human serum albumin. Demonstration of a glycation-induced anionic heterogeneity. J Chromatogr 476: 467–475
Rennke HFG, Cotran RS, Venkatachalam MA (1975) Role of molecular charge in glomerular permeability: tracer studies with cationized ferritins. J Cell Biol 67: 638–643
Deckert T, Feldt-Rasmussen B, Djurup R, Deckert M (1988) Glomerular size and charge selectivity in insulin-dependent diabetes mellitus. Kidney Int 33: 100–106
Bent-Hansen L (1991) Initial plasma disappearance and tissue uptake of 131I-albumin in normal rabbits. Microvasc Res 41: 345–356
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bent-Hansen, L., Feldt-Rasmussen, B., Kverneland, A. et al. Plasma disappearance of glycated and non-glycated albumin in Type 1 (insulin-dependent) diabetes mellitus: evidence for charge dependent alterations of the plasma to lymph pathway. Diabetologia 36, 361–363 (1993). https://doi.org/10.1007/BF00400242
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00400242