Abstract
Concentrations of adenine and pyridine nucleotides and the associated charge values were examined in extracts of mycelium of Penicillium notatum during vegetative growth and reproductive development promoted by the addition of Ca2+ (10 mmol dm-3). The significant increase in adenylate energy charge promoted by Ca2+ was due to a fall in intracellular AMP and a concomitant rise in ATP concentration. Intracellular concentrations of NADH and NAD fell within 1 h of the addition of Ca2+. The catabolic reduction charge was unchanged by Ca2+ whilst the anabolic reduction charge increased in Ca2+-induced mycelium due to lowered intracellular NADP concentration. Reduced concentration of NADPH in Ca2+-induced mycelium, relative to the vegetative controls, lowered the phosphorylated nucleotide fraction. The results are discussed in relation to metabolic economy during morphogenesis in P. notatum.
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References
Afanasieva TP, Filippovich SY, Sokolowsky VY & Kritsky MS (1982) Developmental regulation of NAD+ kinase in Neurospora crassa. Arch. Microbiol. 133: 307–311
Berridge MJ (1984) Inositol trisphosphate and diacylglycerol as second messengers. Biochem. J. 220: 345–360
Brody S (1972) Regulation of pyridine nucleotide levels and ratios in Neurospora crassa. J. Biol. Chem. 247: 6013–6017
Bruinenberg PM, Van Dijken JP & Scheffers WA (1983a) A theoretical analysis of NADPH production and consumption in yeasts. J. Gen. Microbiol. 129: 953–964
Bruinenberg PM, Van Dijken JP & Scheffers WA (1983b) An enzyme analysis of NADPH production and consumption in Candida utilis. J. Gen. Microbiol. 129: 965–971
Bruinenberg PM, Van Dijken JP, Kuenen JG & Scheffers WA (1985) Oxidation of NADH and NADPH by mitochondria from the yeast Candida utilis. J. Gen. Microbiol. 131: 1043–1051
Bygrave FL (1977) Mitochondrial calcium transport. Current Topics in Bioenergetics. 6: 259–318
Cheung WY (1980) Calmodulin plays a pivotal role in cellular regulation. Science 207: 19–27
Dahl JS & Dahl CE (1985) Stimulation of cell proliferation and polyphosphoinositide metabolism in Saccharomyces cerevisiae GL7 by ergosterol. Biochim. Biophys. Res. Commun. 133: 844–850
Delmer DP & Brody S (1975) Circadian rhythms in Neurospora crassa: oscillation in the level of an adenine nucleotide. J. Bacteriol. 121: 548–553
Fuhrer L, Kubicek CP & Rohr M (1980) Pyridine nucleotide levels in Aspergillus niger. Canad. J. Microbiol. 26: 405–408
Kimmich GA, Randles J & Brand JS (1975) Assay of picomole amounts of ATP, ADP and AMP using the luciferase enzyme system. Anal. Biochem. 69: 187–206
Klingenberg M (1985) Nicotinamide-adenine dinucleotides and dinucleotide phosphates. Endpoint UV methods. In: Bergmeyer HU (Ed) Methods of Enzymatic Analysis, Vol. VIII (pp 251–271) VCH, Weinheim
Knowles CJ (1977) Microbial metabolic regulation by adenine nucleotide pools. Symp. Soc. Gen. Microbiol. 27: 241–283
Kritsky MS, Chernyshova EK, Soboleva IS (1977) Nicotinamide coenzymes at the early stage of light induction of carotenogenesis in Neurospora crassa mycelium. Prikladnya Biokhimia I Mikobiologia 13: 901–905
Pitt D & Barnes JC (1987) Hexose transport during calcium-induced conidiation in Penicillium notatum. Trans. Brit. Mycol. Soc. 89: 359–365
Pitt D & Mosley MJ (1985) Pathways of glucose catabolism and the origin and metabolism of pyruvate during calcium-induced conidiation of Penicillium notatum. Antonie van Leeuwenhoek 51: 365–384
Pitt D & Mosley MJ (1986) Oxidation of carbon sources via the tricarboxylic acid cycle during calcium-induced conidiation of Penicillium notatum. Antonie van Leeuwenhoek 52: 467–482
Pitt DE & Bull AT (1982) The adenine nucleotide composition of growing and stressed cultures of Trichoderma aureoviride. Experiment. Mycol. 6: 41–51
Schmit JC, Fahey RC & Brody S (1975) Initial biochemical events in germination of Neurospora crassa conidia. In: Gerhardt P, Costilow RN & Sadoff HL (Eds) Spores, Vol. VI (pp 112–119) American Society for Microbiology, Washington DC
Talwalkar RT & Lester RL (1973) The response of diphosphoinositide and triphosphoinositide to perturbations of the adenylate energy charge in cells of Saccharomyces cerevisiae. Biochim. Biophys. Acta 306: 412–421
Thompson FM & Atkinson DE (1971) Response of nucleoside diphosphate kinase to the adenylate energy charge. Biochim. Biophys. Res. Commun. 45: 1581–1585
Ugalde UO & Pitt D (1988) Properties of mitochondria from Penicillium cyclopium and their response to calcium and other divalent cations. Antonie van Leeuwenhoek 54: 221–228
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Mosley, M.J., Pitt, D. & Barnes, J.C. Adenine and pyridine nucleotide levels during calcium-induced conidiation in Penicillium notatum . Antonie van Leeuwenhoek 56, 191–199 (1989). https://doi.org/10.1007/BF00399982
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DOI: https://doi.org/10.1007/BF00399982