Summary
Hybridoma clones were produced by fusion of splenocytes from glioma-immunized hosts and the X63-Ag8.653 mouse myeloma line and Y3-Ag.1.2.3. rat myeloma line. Oncornavirus particles were found in all clones descending from the mouse myeloma line. No virus particles could be found in either the spleens of immunized Balb/c mice and Fischer rats or in the rat myeloma line and the hybridomas derived from it.
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References
Bartal AH, Feit C, Erlandson R, Hirshaut Y (1982) The presence of vital particles in hybridoma clones secreting monoclonal antibodies. N Engl J Med 306:1423
Deinhardt F (1980) Biology of primate retroviruses. In: Klein G (ed) Viral oncology. Raven Press, New York (pp 357–398)
Dulbecco R (1975) Mechanisms of virus-induced oncogenesis. In: Shein HM (ed) Interaction of brain cells and viruses. MIT Press, Cambridge, Massachusetts (pp 1057–1065)
Galfré G, Milstein C, Wright B (1979) Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG. Nature 277:131–133
Kearny JF, Radbruch A, Liesegang B, Rajewsky K (1979) A new mouse myeloma cell line that has lost immunologlobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol 123:1548–1550
Mondal H, Hofschneider PH (1982) Isolation and chracterization of retrovirus-like elements from normal human fetuses. Int J Cancer 30:281–287
Rozman C, Vilella R, Vives J (1982) Viral particles in hybridoma clones secreting monoclonal antibodies. Lancet II:445
Stavrou D, Hultén M, Anzil AP, Bilzer Th (1980) The humoral antibody response of rats immunized with chemically modified syngeneic brain cells and glioma cells. Int J Cancer 26:629–637
Stavrou D, Anzil AP (1982) Neoplastic transformation of low-passage human fetal brain cells: cultured characteristics and tumorigenicity of the virus-producing permanent cell line. Verh Dtsch Krebs Ges 3:549
Starvou D, Süss C, Bilzer T, Kummer U, de Tribolet N (1983) Monoclonal antibodies reactive with glioma cell lines derived from experimental brain tumors. Eur J Cancer Res Clin Oncol (in press)
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This work was supported by the W. Sander-Foundation (Grant Sta-81.007.1) and the C. Bohnewand Fund of Cancer Research, University of Munich
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Stavrou, D., Bilzer, T., Tsangaris, T. et al. Presence and absence of virus particles in hybridomas secreting monoclonal antibodies against gliomas. J Cancer Res Clin Oncol 106, 77–80 (1983). https://doi.org/10.1007/BF00399903
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DOI: https://doi.org/10.1007/BF00399903