Skip to main content

Degradation of pyrimidine ribonucleosides by Pseudomonas aeruginosa

Antonie van Leeuwenhoek volume 69, pages 331–335 (1996)Cite this article

Abstract

Pyrimidine ribonucleoside degradation in the human pathogen Pseudomonas aeruginosa ATCC 15692 was investigated. Either uracil, cytosine, 5-methylcytosine, thymine, uridine or cytidine supported P. aeruginosa growth as a nitrogen source when glucose served as the carbon source. Using thin-layer chromatographic analysis, the enzymes nucleoside hydrolase and cytosine deaninase were shown to be active in ATCC 15692. Compared to (NH4)2SO4-grown cells, nucleoside hydrolase activity in ATCC 15692 approximately doubled after growth on 5-methylcytosine as a nitrogen source while its cytosine deaminase activity increased several-fold after growth on the pyrimidine bases and ribonucleosides examined as nitrogen sources. Regulation at the level of protein synthesis by 5-methylcytosine was indicated for nucleoside hydrolase and cytosine deaminase in P. aeruginosa.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

References

  • Bachmann BJ (1972) Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol. Rev. 36: 525–557

    PubMed  CAS  Google Scholar 

  • Beck CF, Ingraham JL, Neuhard J & Thomassen E (1972) Metabolism of pyrimidines and pyrimidine nucleosides by Salmonella typhimurium. J. Bacteriol. 110: 219–228

    PubMed  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye-binding. Anal. Biochem. 72: 248–254

    Article  PubMed  CAS  Google Scholar 

  • Chu C-P & West TP (1990) Pyrimidine ribonucleoside catabolism in Pseudomonas fluorescens biotype A. Antonie van Leeuwenhoek 57: 253–257

    Article  PubMed  CAS  Google Scholar 

  • De Vos P & De Ley J (1983) Intra-and intergeneric similarities of Pseudomonas and Xanthomonas rioosomal ribonucleic acid cistrons. Int. J. Syst. Bacteriol. 33: 487–509

    Article  Google Scholar 

  • Fink K, Cline RE & Fink RM (1963) Paper chromatography of several classes of compounds: correlated Rf values in a variety of solvent systems. Anal. Chem. 35: 389–398

    Article  CAS  Google Scholar 

  • Gilardi G (1991) Pseudomonas and related genera. In: Balow A, Hausler WJ Jr, Hermann KL, Isenberg HD & Shadomy HJ (Eds) Manual of Clinical Microbiology (pp 429–441) American Society for Microbiology, Washington, DC

    Google Scholar 

  • Holloway BW (1955) Genetic recombination in Pseudomonas aeruginosa. J. Gen. Microbiol. 13: 572–581

    PubMed  CAS  Google Scholar 

  • Kim S & West TP (1991) Pyrimidine catabolism in Pseudomonas aeruginosa. FEMS Microbiol. Lett. 77: 175–180

    Article  CAS  Google Scholar 

  • Kirchner JG (1978) Thin-layer chromatography. In: Perry ES (Ed) Techniques of Chemistry. Vol. 14 (pp 193–264) John Wiley & Sons, New York

    Google Scholar 

  • O'Donovan GA & Neuhard J (1970) Pyrimidine metabolism in microorganisms. Bacteriol. Rev. 34: 278–343

    PubMed  Google Scholar 

  • Sakai T, Yu T, Taniguchi K & Omata S (1975) Purification of cytosine deaminase from Pseudomonas aureofaciens. Agric. Biol. Chem. 39: 2015–2020

    CAS  Google Scholar 

  • Sakai T, Yu T & Omata S (1976) Distribution of enzymes related to cytidine degradation in bacteria. Agric. Biol. Chem. 40: 1893–1895

    CAS  Google Scholar 

  • Terada M, Tatibana M & Hayaishi O (1967) Purification and properties of nucleoside hydrolase from Pseudomonas fluorescens. J. Biol. Chem. 242: 5578–5585

    PubMed  CAS  Google Scholar 

  • West TP (1989) Isolation and characterization of thymidylate synthetase mutants of Xanthomonas maltophilia. Arch. Microbiol. 151: 220–222

    Article  PubMed  CAS  Google Scholar 

  • West TP (1991) Pyrimidine base and ribonucleoside utilization by the Pseudomonas alcaligenes group. Antonie van Leeuwenhoek 59: 263–268

    Article  PubMed  CAS  Google Scholar 

  • West TP (1994) Pyrimidine ribonucleoside catabolic enzyme activities of Pseudomonas pickettii. Antonie van Leeuwenhoek 66: 307–312

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Thomas P. West

    Present address: Olson Biochemistry Laboratories, Department of Chemistry and Biochemistry, South Dakota Dakota State University, 57007, Brookings, SD, USA

Authors and Affiliations

  1. Department of Biological Sciences, University of Southern Mississippi, 39406, Hattiesburg, MS, USA

    Thomas P. West

Authors
  1. Thomas P. West
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

West, T.P. Degradation of pyrimidine ribonucleosides by Pseudomonas aeruginosa . Antonie van Leeuwenhoek 69, 331–335 (1996). https://doi.org/10.1007/BF00399622

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00399622

Key words

  • pyrimidine
  • nucleoside hydrolase
  • cytosine deaminase
  • Pseudomonas aeruginosa
  • 5-methylcytosine