Pyrimidine ribonucleoside degradation in the human pathogen Pseudomonas aeruginosa ATCC 15692 was investigated. Either uracil, cytosine, 5-methylcytosine, thymine, uridine or cytidine supported P. aeruginosa growth as a nitrogen source when glucose served as the carbon source. Using thin-layer chromatographic analysis, the enzymes nucleoside hydrolase and cytosine deaninase were shown to be active in ATCC 15692. Compared to (NH4)2SO4-grown cells, nucleoside hydrolase activity in ATCC 15692 approximately doubled after growth on 5-methylcytosine as a nitrogen source while its cytosine deaminase activity increased several-fold after growth on the pyrimidine bases and ribonucleosides examined as nitrogen sources. Regulation at the level of protein synthesis by 5-methylcytosine was indicated for nucleoside hydrolase and cytosine deaminase in P. aeruginosa.
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Bachmann BJ (1972) Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol. Rev. 36: 525–557
Beck CF, Ingraham JL, Neuhard J & Thomassen E (1972) Metabolism of pyrimidines and pyrimidine nucleosides by Salmonella typhimurium. J. Bacteriol. 110: 219–228
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye-binding. Anal. Biochem. 72: 248–254
Chu C-P & West TP (1990) Pyrimidine ribonucleoside catabolism in Pseudomonas fluorescens biotype A. Antonie van Leeuwenhoek 57: 253–257
De Vos P & De Ley J (1983) Intra-and intergeneric similarities of Pseudomonas and Xanthomonas rioosomal ribonucleic acid cistrons. Int. J. Syst. Bacteriol. 33: 487–509
Fink K, Cline RE & Fink RM (1963) Paper chromatography of several classes of compounds: correlated Rf values in a variety of solvent systems. Anal. Chem. 35: 389–398
Gilardi G (1991) Pseudomonas and related genera. In: Balow A, Hausler WJ Jr, Hermann KL, Isenberg HD & Shadomy HJ (Eds) Manual of Clinical Microbiology (pp 429–441) American Society for Microbiology, Washington, DC
Holloway BW (1955) Genetic recombination in Pseudomonas aeruginosa. J. Gen. Microbiol. 13: 572–581
Kim S & West TP (1991) Pyrimidine catabolism in Pseudomonas aeruginosa. FEMS Microbiol. Lett. 77: 175–180
Kirchner JG (1978) Thin-layer chromatography. In: Perry ES (Ed) Techniques of Chemistry. Vol. 14 (pp 193–264) John Wiley & Sons, New York
O'Donovan GA & Neuhard J (1970) Pyrimidine metabolism in microorganisms. Bacteriol. Rev. 34: 278–343
Sakai T, Yu T, Taniguchi K & Omata S (1975) Purification of cytosine deaminase from Pseudomonas aureofaciens. Agric. Biol. Chem. 39: 2015–2020
Sakai T, Yu T & Omata S (1976) Distribution of enzymes related to cytidine degradation in bacteria. Agric. Biol. Chem. 40: 1893–1895
Terada M, Tatibana M & Hayaishi O (1967) Purification and properties of nucleoside hydrolase from Pseudomonas fluorescens. J. Biol. Chem. 242: 5578–5585
West TP (1989) Isolation and characterization of thymidylate synthetase mutants of Xanthomonas maltophilia. Arch. Microbiol. 151: 220–222
West TP (1991) Pyrimidine base and ribonucleoside utilization by the Pseudomonas alcaligenes group. Antonie van Leeuwenhoek 59: 263–268
West TP (1994) Pyrimidine ribonucleoside catabolic enzyme activities of Pseudomonas pickettii. Antonie van Leeuwenhoek 66: 307–312
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West, T.P. Degradation of pyrimidine ribonucleosides by Pseudomonas aeruginosa . Antonie van Leeuwenhoek 69, 331–335 (1996). https://doi.org/10.1007/BF00399622
- nucleoside hydrolase
- cytosine deaminase
- Pseudomonas aeruginosa