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Techniques for genetic engineering in mycobacteria

Alternative host strains, DNA-transfer systems and vectors

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Abstract

The study of mycobacterial genetics has experienced quick technical developments in the past ten years, despite a relatively slow start, caused by difficulties in accessing these recalcitrant species. The study of mycobacterial pathogenesis is important in the development of new ways of treating tuberculosis and leprosy, now that the emergence of antibiotic-resistant strains has reduced the effectiveness of current therapies. The tuberculosis vaccine strain M. bovis BCG might be used as a vector for multivalent vaccination. Also, non-pathogenic mycobacterial strains have many possible biotechnological applications. After giving a historical overview of methods and techniques, we will discuss recent developments in the search for alternative host strains and DNA transfer systems. Special attention will be given to the development of vectors and techniques for stabilizing foreign DNA in mycobacteria.

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  1. Division of Industrial Microbiology, Department of Food Science, Agricultural University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands

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Hermans, J., de Bont, J.A.M. Techniques for genetic engineering in mycobacteria. Antonie van Leeuwenhoek 69, 243–256 (1996). https://doi.org/10.1007/BF00399613

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