Abstract
The genetics of denitrification is a relatively unexplored area that has great promise. Species of Pseudomonas are probably best suited for study because they are widely found among natural denitrifying populations and are quite readily amenable to genetic analysis. The techniques for mutagenesis and for the exchange of chromosomal genes to characterize mutant strains have been well-developed in P. aeruginosa and are being developed in P. stutzeri. Mutants defective in the denitrification of nitrate, nitrite, and nitrous oxide are now available and will aid in describing the catalytic and regulatory elements of the denitrification pathway.
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Anagnostopoulos, C. and Spizizen, J. 1961. Requirements for transformation in Bacillus subtilis. — J. Bacteriol. 81: 741–746.
Beringer, J. E., Beynon, J. L., Buchanan-Wollaston, A. V. and Johnston, A. W. B. 1978. Transfer of the drug- resistance transposon Tn5 to Rhizobium. — Nature 276: 633–634.
Carlson, C. A. and Ingraham, J. L. 1981. The physiological genetics of denitrification in Pseudomonas, p. 429–443. In J. M. Lyons, R. C. Valentine, D. A. Phillips, D. W. Rains, and R. C. Huffaker (eds), Genetic engineering of symbiotic nitrogen fixation and conservation of fixed nitrogen. — Plenum Pulbishing Corporation, New York.
Cohn, W., and Crawford, I. P. 1976. Regulation of enzyme synthesis in the tryptophan pathway of Acinetobacter calcoaceticus. — J. Bacteriol. 127: 367–379.
Focht, D. D. and Joseph, H. 1974. Degradation of 1,1-diphenylethylene by mixed cultures. —Can. J. Microbiol. 20: 631–635.
Gamble, T. N., Betlach, M. R. and Tiedje, J. M. 1977. Numerically dominant denitrifying bacteria from world soils. — Appl. Environ. Microbiol. 33: 926–939.
Gorini, L. and Kataja, E. 1965. Suppression activated by streptomycin and related antibiotics in drug sensitive strains. — Biochem. Biophys. Res. Commun. 18: 656–663.
Haas, D., and Holloway, B. W. 1976. R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. — Molec. Gen. Genet. 144: 243–251.
Herman, N. J. and Juni, E. 1974. Isolation and characterization of a generalized transducing bacteriophage for Acinetobacter. — J. Virol. 13: 46–52.
Hoch, J. A. 1978. Developmental genetics at the beginning of a new era. p. 119–121. In G. Chambliss and J. C. Vary (eds). Spores VII. — American Society for Microbiology, Washington, D.C.
Holloway, B. W., Krishnapillai, V. and Morgan, A. F. 1979. Chromosomal genetics of Pseudomonas. Microbiol. Rev. 43: 73–102.
Ingraham, J. L. 1981. Microbiology and genetics of denitrifiers, p. 45–65. In C. C. Delwiche, (ed.), Denitrification, nitrificiation and atmospheric nitrous oxide. — John Wiley and Sons, Inc.
Juni, E. and Janek, A. 1969. Transformation of Acinetobacter calcoaceticus. — J. Bacteriol. 98: 281–288.
Kleckner, N., Roth, J. and Botstein, D. 1977. Genetic engineering in vivo using translocatable drug-resistance elements. — J. Mol. Biol. 116: 125–129.
Mindich, L., Cohen, J. and Weisburd, M. 1976. Isolation of nonsense suppressor mutants in Pseudomonas. — J. Bacteriol. 126: 177–182.
Olsen, R. H., Siak, J.-S. and Gray, R. H. 1974. Characteristics of PRD1, a plasmid-dependent broad host range DNA bacteriophage. — J. Virol. 14: 689–699.
Palleroni, N. J., Kunisawa, R., Contopoulou, R. and Doudoroff, M. 1973. Nucleic acid homologies in the genus Pseudomonas. — Int. J. Syst. Bacteriol. 23: 333–339.
Payne, W. J. 1981. Denitrification. — John Wiley and Sons, Baltimore.
Royle, P. L., Matsumoto, H. and Holloway, B. W. 1981. Genetic circularity of the Pseudomonas aeruginosa PAO chromosome. — J. Bacteriol. 145: 145–155.
St. John., R. T. and Hollocher, T. C. 1977. Nitrogen-15 tracer studies on the pathway of denitrification in Pseudomonas aeruginosa. — J. Biol. Chem. 252: 212–218.
Sato, M., Staskawicz, B. J., Panopoulos, N. J., Peters, S. and Honma, M. 1981. A host-dependent hybrid plasmid suitable as a suicidal carrier for transposable elements. — Plasmid 6: 325–331.
Sias, S. R. and Ingraham, J. L. 1979. Isolation and analysis of mutants of Pseudomonas aeruginosa unable to assimilate nitrate. — Arch. Microbiol. 122: 263–270.
Sias, S. R., Stouthamer, A. H. and Ingraham, J. L. 1980. Assimilatory and dissimilatory nitrate reductases in Pseudomonas aeruginosa are encoded by different genes. — J. Gen. Microbiol. 118: 229–234.
Smith, H. O., Danner, D. B. and Deich, R. A. 1981. Genetic transformation, p. 41–68. In E. E. Snell, P. D. Boyer, A. Meister and C. C. Richardson (eds), Annu. Rev. Biochem., Vol. 50. —Annual Reviews, Inc.
Stouthamer, A. H. 1976. Biochemistry and genetics of nitrate reductase in bacteria, p. 315–375. In A. H. Rose and D. W. Tempest (eds), Advances in microbial physiology, Vol. 14. — Academic Press, New York.
Towner, K. J. 1978. Chromosome mapping in Acinetobacter calcoaceticus. — J. Gen. Microbiol. 104: 175–180.
Van Hartingsveldt, J. and Stouthamer, A. H. 1973. Mapping and characterization of mutants of Pseudomonas aeruginosa affected in nitrate respiration in aerobic or anaerobic growth. — J. Gen. Microbiol. 74: 97–106.
Van Hartingsveldt, J., Marinus, M. G. and Stouthamer, A. H. 1971. Mutants of Pseudomonas aeruginosa blocked in nitrate or nitrite dissimilation. — Genetics 67: 469–482.
Van Neil, C. B. and Allen, M. B. 1952. A note on Pseudomonas stutzeri. — J. Bacteriol. 64: 413–422.
Warren, R. A. J. 1979. Amber suppressor mutations in Pseudomonas acidovorans. — J. Bacteriol. 137: 1053–1055.
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Financial support has been provided in part by National Science Foundation grant AER77-07301 and U.S. Department of Agriculture grant 59-2063-1-1627-0.
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Carlson, C.A. The physiological genetics of denitrifying bacteria. Antonie van Leeuwenhoek 48, 555–567 (1983). https://doi.org/10.1007/BF00399541
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DOI: https://doi.org/10.1007/BF00399541