Abstract
The amount of total histone and separate groups of histone fractions were determined in isolated individual nuclei of the cricket spermatogonia and spermatocytes by the use of quantitative interference microscopy before and after the stepwise histone extraction with hydrochloric acid at pH 2.2, 1.5 and 0.7. It is established that during spermatogonial interphase the amount of the total histone per nucleus is doubled from approximately 7 pg (2c) to about 13–14 pg (4c). At the end of premeiotic interphase the amount of total histone per nucleus reaches about 11 pg (about 3c). At the end of pachytene the amount of histone per nucleus reaches about 17–18 pg (5c). —The additional amount of histone per pachytene nucleus (about 3 pg) was extracted from the isolated pachytene nuclei at pH 2.2 together with FI histone. This part of histone probably corresponds to histone of meiosis originally described by Sheridan and Stern (1967) and confirmed in our laboratory (Strokov et al., in preparation).
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Liapunova, N.A., Babadjanian, D.P. A quantitative study of histones in meioeytes I. Investigation of the histone amount in cricket spermatogenesis by interference microscopy. Chromosoma 40, 387–399 (1973). https://doi.org/10.1007/BF00399430
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DOI: https://doi.org/10.1007/BF00399430