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Phenylalanine hydroxylase activity in a mutant of Geotrichum candidum

  • Biochemistry
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Abstract

A mutant of Geotrichum candidum was isolated with a tyrosine requirement which could be satisfied by l-tyrosine or l-phenylalanine. l-Phenylalanine is converted by cell suspensions to l-tyrosine, which can be detected in the growth medium. The incorporation of the tyrosine into cell protein is described.

l-Phenylalanine is converted to tyrosine by cell-free extracts with a requirement for some dialysable components.

The adaptation of intact cells to phenylalanine metabolism is also described.

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References

  • Dagley, S., Fewster, M. E. and Happold, F. C. 1953. The bacterial oxidation of aromatic compounds.— J. Gen. Microbiol. 8: 1–7.

    PubMed  Google Scholar 

  • Friedman, P. A. and Kaufman, S. 1971. A study of the development of phenylalanine hydroxylase in fetuses of several mammalian species.— Arch. Biochem. Biophys. 146: 321–326.

    PubMed  Google Scholar 

  • Friedrich, B. und Schlegel, H. G. 1972. Die Hydroxylierung von Phenylalanin durch Hydrogenomonas eutropha H 16.— Arch. Mikrobiol. 83: 17–31.

    PubMed  Google Scholar 

  • Guroff, G. and Abramowitz, A. 1967. A simple radioisotope assay for phenylalanine hydroxylase. — Analyt. Biochem. 19: 548–555.

    PubMed  Google Scholar 

  • Guroff, G. and Ito, T. 1965. Phenylalanine hydroxylation by Pseudomonas species (ATCC 11299a).— J. Biol. Chem. 240: 1175–1184.

    Google Scholar 

  • Guroff, G. and Rhoads, C. A. 1967. Phenylalanine hydroxylase from Pseudomonas species (ATCC 11299a).— J. Biol. Chem. 242: 3641–3645.

    PubMed  Google Scholar 

  • Kaufman, S. 1962. Aromatic hydroxylations, p. 129–180. In Oxygenases (O. Hayashi, ed.)—Academic Press Inc. N.Y.

    Google Scholar 

  • Knox, W. E. and LeMay-Knox, M. 1951. The oxidation in liver of l-tyrosine to acetoacetate through p-hydroxyphenyolpyruvate and homogentisic acid.— Biochem. J. 49: 686–693.

    PubMed  Google Scholar 

  • Kunita, N. 1956. Evidence for the alternative pathways for the oxidation of phenylalanine by Pseudomonas fluorescens.— Med. J. Osaka Univ. 7: 203–215.

    Google Scholar 

  • LaDu, B. N. and Zannoni, V. G. 1955. The tyrosine oxidation system of liver. II. Oxidation of p-hydroxyphenylpyruvic acid to homogentisic acid.— J. Biol. Chem. 217: 777–787.

    PubMed  Google Scholar 

  • Malling, H. V. and De Serres, F. J. 1970. Genetic effects of N-methyl-N′-nitro-N-nitrosoguanidine in Neurospora crassa.— Molec. Gen. Genetics 106: 195–207.

    Google Scholar 

  • Miller, D. A. and Simmonds, S. 1957. Phenylalanine and tyrosine metabolism in E. coli strain K-12.— Science 126: 445–446.

    PubMed  Google Scholar 

  • Schwinck, J. and Adams, E. 1959. Aromatic biosynthesis. XVI. Aromatization of prephenic acid to p-hydroxyphenylpyruvic acid, a step in tyrosine biosynthesis in Escherichia coli.—Biochim. Biophys. Acta 36: 102–117.

    Article  PubMed  Google Scholar 

  • Tourian, A. 1973. Phenylalanine hydroxylase activity in foetal hepatic organ culture.—Biochim. Biophys. Acta 309: 44–49.

    PubMed  Google Scholar 

  • Udenfriend, S. and Cooper, J. R. 1953. Assay of l-phenylalanine as phenethylamine after enzymatic decarboxylation: application to isotopic studies.— J. Biol. Chem. 203: 953–960.

    PubMed  Google Scholar 

  • Waalkes, T. P. and Udenfriend, S. 1957. A fluorometric method for the estimation of tyrosine in plasma and tissues.— J. Lab. Clin. Med. 50: 733–736.

    PubMed  Google Scholar 

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  1. Department of Industrial Microbiology, University College, Ardmore, Stillorgan Rd., 4, Dublin, Ireland

    J. J. McEvoy

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  1. J. J. McEvoy
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McEvoy, J.J. Phenylalanine hydroxylase activity in a mutant of Geotrichum candidum . Antonie van Leeuwenhoek 40, 409–416 (1974). https://doi.org/10.1007/BF00399353

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