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Carnosinase: its presence in Pseudomonas aeruginosa

  • Biochemistry
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Abstract

A carnosine-hydrolyzing bacterium was isolated from soil by aerobic enrichment and identified as Pseudomonas aeruginosa. Cell-free extracts of this organism and also of other Ps. aeruginosa strains contained carnosinase. The activity was measured by either a radioassay of a fluorometric assay.

Carnosinase is an inducible enzyme. Although induction was achieved by its substrate, carnosine, the best induction was obtained by β-alanine, a product of the enzyme reaction.

Some general properties of the crude enzyme were determined.

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Author notes

  1. H. C. J. Ketelaars

    Present address: Department of Pharmacology, University of Nijmegen, Nijmegen, The Netherlands

Authors and Affiliations

  1. Laboratory of Microbiology, Faculty of Science, University of Nijmegen, Nijmegen, The Netherlands

    C van der Drift & H. C. J. Ketelaars

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  1. C van der Drift
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van der Drift, C., Ketelaars, H.C.J. Carnosinase: its presence in Pseudomonas aeruginosa . Antonie van Leeuwenhoek 40, 377–384 (1974). https://doi.org/10.1007/BF00399349

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  • DOI: https://doi.org/10.1007/BF00399349

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