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Purification of an onco-fetal antigen from mammary carcinoma cells

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Summary

Mammary carcinoma cell membranes were solubilized by a zwitterionic detergent, 3-(3-cholamidopropyl)-dimethyl-ammonio)-propanesulfonate (CHAPS). The solubilized membrane components were resolved by high pressure liquid chromatography (HPLC) using a styrogel protein column. One of the fractions was found to react specifically with anti-F9-antiserum in a solid-phase immunosorbent assay. Following recycling this fraction was purified to homogeneity and had an N-terminal amino acid residue of lysine. Its molecular weight was estimated to be 15,000 daltons.

No comparable fraction could be detected in the solubilized membrane components of nonmalignant mammary tissue.

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Authors and Affiliations

  1. Abteilung Medizinische Biochemie, Universitäts-Frauenklinik Kiel, Hegewischstrasse 4, D-2300, Kiel, Federal Republic of Germany

    A. B. Czuppon

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  1. A. B. Czuppon
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Czuppon, A.B. Purification of an onco-fetal antigen from mammary carcinoma cells. J Cancer Res Clin Oncol 110, 252–254 (1985). https://doi.org/10.1007/BF00399285

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  • DOI: https://doi.org/10.1007/BF00399285

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