, Volume 84, Issue 2, pp 207–219 | Cite as

Cytogenetic analysis of the 2B3-4-2B11 Region of the X chromosome of Drosophila melanogaster

II. Changes in 20-OH ecdysone puffing caused by genetic defects of puff 2B5
  • E. S. Belyaeva
  • I. E. Vlassova
  • Z. M. Biyasheva
  • V. T. Kakpakov
  • G. Richards
  • I. F. Zhimulev


Larvae homozygous or hemizygous for the l(l) t435 mutation located within the early ecdysteroid puff 2B5, or carrying a deletion of the 2B5 band, die at the end of the third larval instar. In the salivary gland chromosomes of these larvae only intermoult puffs are detected. If these salivary glands are incubated in vitro with 20-OH ecdysone for 6 h the intermoult puff 68 C remains large, some early puffs (74EF and 75B) are induced to 30–40% of their normal size, other early (63F) and all late puffs (62E, 78D, 82F and 63E) are not induced at all. Puff 2B5 reaches its normal size but does not regress after 6h incubation with 20-OH ecdysone, as it does in normal stocks. The data obtained in this study show the existence of a locus (or loci) in the band (puff) 2B5 which is necessary for the normal response of the salivary gland chromosomes to the hormone 20-OH ecdysone.


Salivary Gland Developmental Biology Normal Response Larval Instar Cytogenetic Analysis 
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  1. Aizenzon, M.G., Belyaeva, E.S.: Genetic loci in the 2A-B interval of Drosophila melanogaster X chromosome. Dros. Inf. Serv. (in press, 1981)Google Scholar
  2. Aizenzon, M.G., Belyaeva, E.S., Zhimulev, I.F.: Cytegenetic analysis of Drosophila melanogaster X chromosome region 2 B1.2-2B9.10.IV. Phenocrytic and effective lethal phases of mutants. Genetika (U.S.S.R.) (in press, 1981)Google Scholar
  3. Ashburner, M.: Patterns of puffing activity in the salivary gland chromosomes of Drosophila. VI. Induction by ecdysone in salivary glands of Drosophila melanogaster cultured in vitro. Chromosoma (Berl.), 38, 255–281 (1972)Google Scholar
  4. Ashburner, M.: Sequential gene activation by ecdysone in polytene chromosomes of Drosophila melanogaster. I. Dependence upon hormone concentration. Devel. Biol., 35, 47–61 (1973)Google Scholar
  5. Ashburner, M., Bonner, J.J.: The induction of gene activity in Drosophila by heat shock. Cell 17, 241–254 (1979)Google Scholar
  6. Ashburner, M., Chihara, C., Meltzer P., Richards, G.: On the temporal control of puffing activity in polytene chromosomes. Cold Spr. Har. Symp. quant. Biol. 38, 655–662 (1974)Google Scholar
  7. Belyaeva, E.S., Aizenzon, M.G., Semeshin, V.F., Kiss, I., Koczka, K., Baricheva, E.M., Gorelova, T.D., Zhimulev, I.F.: Cytogenetic analysis of the 2B3-4-2B11 region of the X chromosome of Drosophila melanogaster. I. Cytology of the region and mutant complementation groups. Chromosoma (Berl.), 81, 281–306 (1980)Google Scholar
  8. Belyaeva, E.S., Aizenzon, M.G., Kiss, I., Gorelova, T.D., Pak, S.D., Umbetova, G.H., Kramers, P.G., Zhimulev, I.F.: Mutations and chromosome rearrangements in the 1E3-4-2B18 region of Drosophila melanogaster X chromosome. Dros. Inf. Serv. (in press, 1981)Google Scholar
  9. Biyasheva, Z.M., Zhimulev, I.F., Kakpakov, V.T., Richards, G., Aizenzon, M.G., Belyaeva, E.S.: Two closely linked loci controlling ecdysone dependent puffing in Drosophila melanogaster. Dokl. Acad. Nauk. U.S.S.R. 256, 983–986 (1981)Google Scholar
  10. Boyd, M., Ashburner, M.: The hormonal control of salivary gland secretion in Drosophila melanogaster: studies in vitro. J. Insect Physiol. 23, 517–523 (1977)Google Scholar
  11. Holden, J.J., Ashburner, M.: Patterns of puffing activity in the salivary and prothoracic gland chromosomes of a dominant temperature sensitive lethal of Drosophila melanogaster. Chromosoma (Berl.), 68, 205–227 (1978)Google Scholar
  12. Kiss, I., Molnar, I.: Metamorphic changes of wild type and mutant Drosophila tissues induced by 20-hydroxyecdysone in vitro. J. Insect Physiol., 26, 391–401 (1980)Google Scholar
  13. Kiss, I., Bencze, G., Fodor, A., Szabad, J., Fristrom, J.: Prepupal-larval mosaics in Drosophila melanogaster. Nature (Lond.) 262, 136–138 (1976)Google Scholar
  14. Kiss, I., Szabad, J., Belyaeva, E.S., Zhimulev, I.F., Major, J.: Genetic and developmental analysis of mutants in an early ecdysone inducible puffing region in Drosophila melanogaster. In: Development and neurobiology of Drosophila (O. Siddiqi, P. Babu, L.M. Hall and J.C. Hall, eds.), pp. 163–181. New York, London: Plenum Press 1980Google Scholar
  15. Poels, C.L.M.: Mucopolysaccharide secretion from Drosophila salivary gland cells as a consequence of hormone induced gene activity. Cell Different. 1, 63–78 (1972)Google Scholar
  16. Poluektova, E.V., Mitrafanov, V.G., Kakpakov, V.T.: Effect of insect hormones on chromosome puffing in salivary glands of Drosophila virilis Sturt. cultivated in vitro. I. Changes of puffing upon cultivation of the glands in C-50 medium. Ontogenez (U.S.S.R) 11, 175–180 (1980)Google Scholar
  17. Richards, G.: Sequential gene activation by ecdysone in polytene chromosomes of Drosophila melanogaster. IV. The mid prepupal period. Develop. Biol. 54, 256–263 (1976a)Google Scholar
  18. Richards, G.: The in vitro induction of puffing in salivary glands of the mutant 1(2)gl of Drosophila melanogaster by ecdysone. Roux' Archiv. Entw. Mech. Org. 179, 339–348 (1976b)Google Scholar
  19. Richards, G.: The radioimmune assay of ecdysteroid titres in Drosophila melanogaster. Molec. cell. Endocrinol. 21, 181–197 (1981)Google Scholar
  20. Zhimulev, I.F., Belyaeva, E.S.: 3H-uridine labelling patterns in the Drosophila melanogaster salivary gland chromosomes X, 2R and 3L. Chromosoma (Berl.), 49, 219–231 (1975)Google Scholar
  21. Zhimulev, I.F., Belyaeva, E.S., Lychev, V.A.: Comparative study of the function of polytene chromosomes in laboratory stocks of Drosophila melanogaster and the 1(3)tl mutant (lethal tumorous larvae). II. Changes of banding pattern and transcriptional activity in the salivary chromosomes of 1(3)tl. Chromosoma (Berl.), 55, 121–136 (1976)Google Scholar
  22. Zhimulev, I.F., Kolesnikov, N.N.: Methods of selection of synchronously developing larvae in Drosophila melanogaster. Ontogenez (U.S.S.R.), 6, 635–639 (1975a)Google Scholar
  23. Zhimulev, I.F., Kolesnikov, N.N.: Synthesis and secretion of mucoprotein glue in the salivary gland of Drosophila melanogaster. Roux' Arch. Entw. Mech. Org. 178, 15–28 (1975b)Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • E. S. Belyaeva
    • 1
  • I. E. Vlassova
    • 1
  • Z. M. Biyasheva
    • 2
  • V. T. Kakpakov
    • 3
  • G. Richards
    • 4
  • I. F. Zhimulev
    • 1
  1. 1.Institute of Cytology and GeneticsSiberian Division of the USSR Academy of ScienceNovosibirskUSSR
  2. 2.Kasakh State UniversityAlma-AtaUSSR
  3. 3.Institute of General Genetics of the USSR Academy of ScienceMoscowUSSR
  4. 4.C.N.R.S. Laboratoire de Genetique Moleculaire des EucaryotesStrasbourg CedexFrance

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