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Chromosoma

, Volume 84, Issue 2, pp 207–219 | Cite as

Cytogenetic analysis of the 2B3-4-2B11 Region of the X chromosome of Drosophila melanogaster

II. Changes in 20-OH ecdysone puffing caused by genetic defects of puff 2B5
  • E. S. Belyaeva
  • I. E. Vlassova
  • Z. M. Biyasheva
  • V. T. Kakpakov
  • G. Richards
  • I. F. Zhimulev
Article

Abstract

Larvae homozygous or hemizygous for the l(l) t435 mutation located within the early ecdysteroid puff 2B5, or carrying a deletion of the 2B5 band, die at the end of the third larval instar. In the salivary gland chromosomes of these larvae only intermoult puffs are detected. If these salivary glands are incubated in vitro with 20-OH ecdysone for 6 h the intermoult puff 68 C remains large, some early puffs (74EF and 75B) are induced to 30–40% of their normal size, other early (63F) and all late puffs (62E, 78D, 82F and 63E) are not induced at all. Puff 2B5 reaches its normal size but does not regress after 6h incubation with 20-OH ecdysone, as it does in normal stocks. The data obtained in this study show the existence of a locus (or loci) in the band (puff) 2B5 which is necessary for the normal response of the salivary gland chromosomes to the hormone 20-OH ecdysone.

Keywords

Salivary Gland Developmental Biology Normal Response Larval Instar Cytogenetic Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • E. S. Belyaeva
    • 1
  • I. E. Vlassova
    • 1
  • Z. M. Biyasheva
    • 2
  • V. T. Kakpakov
    • 3
  • G. Richards
    • 4
  • I. F. Zhimulev
    • 1
  1. 1.Institute of Cytology and GeneticsSiberian Division of the USSR Academy of ScienceNovosibirskUSSR
  2. 2.Kasakh State UniversityAlma-AtaUSSR
  3. 3.Institute of General Genetics of the USSR Academy of ScienceMoscowUSSR
  4. 4.C.N.R.S. Laboratoire de Genetique Moleculaire des EucaryotesStrasbourg CedexFrance

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