Summary
Arginine kinase (AK) is present throughout the life cycle of Drosophila melanogaster, but there is a sharp, transient peak of AK activity during the prepupal period and a second period of elevated activity at the time of eclosion of the adult. Imaginal discs show the greatest increase in AK activity at the prepupal stage of those tissues assayed. The prepupal peak is not seen when the temperature-sensitive ecdysoneless mutant ecd-1 is shifted to 29° C at mid-third instar larval stage. The peak in activity reappears when ecd-1 is either shifted back to 20° C after 60 h at 29° C or is fed 20-hydroxyecdysone. At the restrictive temperature, imaginal discs from ecd-1 larvae progressively lose AK activity, whereas discs from 20-hydroxyecdysone-fed larvae have a marked increase in AK activity at stage P3 of the prepupal period. These data suggest that the prepupal peak is regulated by the hormone 20-hydroxyecdysone.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bainbridge SP, Bownes M (1981) Staging the metamorphosis of Drosophila melanogaster. J Embryol Exp Morphol 66:57–80
Belinshi-Deutsch S, Busson D, Lamkour-Audit C, Porcheron P, Moriniere M, Berreur P (1983) Relations between ecdysteroid levels and pupal development in the ecd-1 temperature-sensitive mutant of Drosophila melanogaster. J Insect Physiol 29:509–514
Blethan SL, Kaplan NO (1968) Characteristics of arthropod arginine kinases. Biochemistry 7:2123–2135
Boyd M, Ashburner M (1977) The hormonal control of salivary gland secretion in Drosophila melanogaster studies in vitro. J Insect Physiol 23:517–525
Cavenar DR, Murtha M, Schonbaum C, Hollar D (1987) Cell autonomous and hormonal control of sex-limited gene expression in Drosophila. In: Law JH (ed) Molecular Entomology. AR Liss, Inc, New York, pp 453–462
Cherbas L, Benes H, Bourouis M, Burtis K, Chae A, Cherbas P, Crosby M, Garfinkel M, Guild G, Hogness D, Jami J, Jones CW, Koehler M, Lepesant J-A, Martin C, Maschat F, Mathers P, Meyerowitz E, Moss R, Pictet R, Rebers J, Richards G, Roux J, Schulz R, Segraves W, Thummel C, Vijayraghavan K (1986) Structural and functional analysis of some moulting hormone-responsive genes from Drosophila. Insect Biochem 16:241–248
Clark WC, Doctor J, Fristrom JW, Hodgetts RB (1986) Differential responses of the dopa decarboxylase gene to 20-OH-ecdysone in Drosophila melanogaster. Dev Biol 114:141–150
Collier GE, James JM (1987) Arginine kinase and pyruvate kinase are coordinately controlled by the 75B “early” gene in Drosophila imaginal discs. Genetics 116s:12
Eckert BS, Koons SJ, Schantz AW, Zobel CR (1980) Association of creatine phosphokinase with the cytoskeleton of cultured mammalian cells. J Cell Biol 86:1–5
Fristrom D, Fristrom JW (1975) The mechanism of evagination of imaginal disks of Drosophila melanogaster. I. General considerations. Dev Biol 43:1–23
Fristrom D, Rickoll WL (1982) The morphogenesis of imaginal discs of Drosophila. In: King RC, Akai H (eds) Insect Ultrastructure. Plenum Press, New York, pp 247–277
Fristrom JW, Logan WR, Murphy C (1973) The synthetic and minimal culture requirements for evagination of imaginal discs of Drosophila melanogaster in vitro. Dev Biol 33:441–456
Fu L-J, Collier GE (1983) Localization of the structural genes for arginine kinase and glyceraldehyde-3-phosphate dehydrogenase in Drosophila melanogaster. Bull Inst Zool Acad Sinica 22:25–35
Garen A, Kauvar L, Lepesant J-A (1977) Roles of ecdysone in Drosophila development. Proc Natl Acad Sci USA 74:5099–5103
Handler AM (1982) Ecdysteroid titers during pupal and adult development in Drosophila melanogaster. Dev Biol 93:73–82
Hodgetts RB, Sage B, O'Connor JD (1977) Ecdysone titers during postembryonic development of Drosophila melanogaster. Dev Biol 60:310–317
Holden JJA, Walker VK, Maroy P, Watson KL, White BN, Gausz J (1986) Analysis of molting and metamorphosis in the ecdysteroid-deficient mutant L(3)3 DTS of Drosophila melanogaster. Dev Genet 6:153–162
James JM, Collier GE (1987) Developmental regulation of arginine kinase in Drosophila imaginal discs. ICSU Short Reports 7:81
James JM, Collier GE (1988) Distribution and genetic basis of arginine kinase in wild type and flightless mutants of Drosophila melanogaster. J Exp Zool 248:185–191
Kraminsky GP, Clark WC, Estelle MA, Gietz RD, Sage BA, O'Connor JD, Hodgetts RB (1980) Induction of translatable mRNA for dopa decarboxylase in Drosophila: An early response to ecdysterone. Proc Natl Acad Sci USA 77:4175–4179
Lowry OH, Rosebrough NJ, Fahr AL, Randall RJ (1951) Protein measurement with Folin-phenol reagent. J Biol Chem 193:265–275
Madhaven MM, Schneiderman HA (1977) Histological analysis of the dynamics of growth of imaginal discs and histoblast nests during larval development of Drosophila melanogaster. Roux's Arch Dev Biol 183:269–305
Mandaron P (1971) Sur le mechanism de l'evagination des disques imaginaux de la Drosophila. Dev Biol 25:581–605
Marsh JL, Wright TRF (1980) Developmental relationship between dopa decarboxylase, dopamine acetyltransferase, and ecdysone in Drosophila. Dev Biol 80:379–387
Morrison JF (1973) Arginine kinase and other invertebrate quanidino kinases. In: Boyer PD (ed) The enzymes vol. VIII, Part A. Academic Press, New York, pp 457–486
Munneke LR, Collier GE (1988) Cytoplasmic and mitochondrial arginine kinase in Drosophila: Evidence for a single gene. Biochem Genet 26:121–131
Redfern CPF, Bownes M (1983) Pleiotropic effects of the ‘ecdysoneless-1’ mutation of Drosophila melanogaster. Mol Gen Genet 189:432–440
Robertson CW (1936) Metamorphosis of Drosophila melanogaster, including an accurately timed account of the principal morphological changes. J Morphol 59:351–399
Schiaffino S, Hanzlikova V (1972) Studies on the effect of degeneration in developing muscle. II. The lysosomal system. J Ultrastruct Res 39:1–14
Seecof RL (1971) Phosphate-buffered saline for Drosophila. Dros Inf Ser 46:113
Storey KB (1977) Purification and characterization of arginine kinase from the mantle muscle of the squid, Symplectoteuthis oualaniensis. Arch Biochem Biophys 179:518–526
Walliman T, Eppenberger HM (1973) Properties of arginine kinase from Drosophila melanogaster. Eur J Biochem 38:180–184
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
James, J.M., Collier, G.E. Hormonally regulated expression of arginine kinase in Drosophila melanogaster . Roux's Arch Dev Biol 198, 474–478 (1990). https://doi.org/10.1007/BF00399058
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00399058