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Circadian rhythms in crassulacean acid metabolism: phase relationships between gas exchange, leaf water relations and malate metabolism in Kalanchoë daigremontiana

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Abstract

Gas exchange, leaf water relations, malate content and phosphoenolpyruvate (PEP) carboxylase activity in crude extracts were examined for circadian rhythmicity in the crassulacean acid metabolism plant Kalanchoë daigremontiana. At low irradiance (20 W m-2) the rhythm in CO2 uptake continued for several days with a period length of approx. 22 h, whereas the transpiration rhythm was no longer apparent after 24 h. This shows that the CO2 rhythm in continuous light (LL) is not under stomatal control. Circadian oscillations in malate content were detectable for up to 72 h in LL but were of much reduced amplitude. This was reflected in the changes in leaf water relations, which quickly damped after transfer to LL. The activity of PEP carboxylase assayed immediately after extraction showed a rhythmicity for at least 18 h, but after 36 h, values from different plants were scattered. We suggest that the CO2-uptake rhythm is primarily the result of endogenous changes in the activity of PEP carboxylase, which competes to varying degrees with ribulose-1,5-bisphosphate carboxylase for CO2.

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Abbreviations

CAM:

crassulacean acid metabolism

LD:

12 h light: 12 h dark cycle

LL:

continuous light

PEP:

phosphoenolpyruvate

P :

turgor pressure

Ψ:

water potential

π:

osmotic pressure

References

  • Bollig, I.C., Wilkins, M.B. (1979) Inhibition of the circadian rhythm of CO2 metabolism in Bryophyllum leaves by cycloheximide and dinitrophenol. Planta 145, 105–112

    Google Scholar 

  • Buchanan-Bollig, I.C. (1984) Circadian rhythms in Kalanchoë: effects of irradiance and temperature on gas exchange and carbon metabolism. Planta 160, 264–271

    Google Scholar 

  • Buchanan-Bollig, I.C., Fischer, A., Kluge, M. (1984) Circadian rhythms in Kalanchoë: the pathway of 14CO2 fixation during prolonged light. Planta 161, 71–80

    Google Scholar 

  • Buchanan-Bollig, I.C., Kluge, M. (1981) Crassulacean acid metabolism (CAM) in Kalanchoë daigremontiana: temperature response of phosphoenolpyruvate (PEP)-carboxylase in relation to allosteric effectors. Planta 152, 181–188

    Google Scholar 

  • Buchanan-Bollig, I.C., Kluge, M., Lüttge, U. (1980) PEP-carboxylase activities and the regulation of CAM: effects of extraction procedures and leaf age. Z. Pflanzenphysiol. 97, 457–470

    Google Scholar 

  • Jones, R., Buchanan, I.C., Wilkins, M.B., Fewson, C.A., Malcolm, A.D.B. (1981) Phosphoenolpyruvate carboxylase from the Crassulacean plant Bryophyllum fedtschenkoi Hamet et Perrier. J. Exp. Bot. 32, 427–441

    Google Scholar 

  • Kluge, M., Böhlke, C., Queiroz, O. (1981a) Crassulacean acid metabolism (CAM) in Kalanchoë: changes in intercellular CO2 concentration during a normal CAM cycle and during cycles in continuous light or darkness. Planta 152, 87–92

    Google Scholar 

  • Kluge, M., Brulfert, J., Queiroz, O. (1981b) Diurnal changes in the regulatory properties of PEP-carboxylase in crassulacean acid metabolism (CAM). Plant Cell Environ. 4, 251–256

    Google Scholar 

  • Kluge, M., Fischer, K. (1967) Über Zusammenhänge zwischen CO2 Austausch und der Abgabe von Wasserdampf durch Bryophyllum daigremontiamum. Planta 77, 212–223

    Google Scholar 

  • Lüttge, U., Ball, E. (1977) Water relation parameters of the CAM plant Kalanchoë daigremontiana in relation to diurnal malate oscillations. Oecologia (Berlin) 31, 85–94

    Google Scholar 

  • Lüttge, U., Ball, E. (1978) Free running oscillations of transpiration and CO2 exchange in CAM plants without a concomitant rhythm of malate levels. Z. Pflanzenphysiol. 90, 69–77

    Google Scholar 

  • Lüttge, U., Kluge, M., Ball, E. (1975) Effects of osmotic gradients on vacuolar malic acid storage. A basic principle in oscillatory behavior of crassulacean acid metabolism. Plant Physiol. 56, 613–616

    Google Scholar 

  • Lüttge, U., Smith, J.A.C., Marigo, G. (1982) Membrane transport, osmoregulation, and the control of CAM. In: Crassulacean acid metabolism, pp. 69–91, Ting, I.P., Gibbs, M., eds. American Society of Plant Physiologists, Maryland

    Google Scholar 

  • Möllering, H. (1974) l-(-)-Malat. Bestimmung mit Malat-Dehydrogenase and Glutamat-Oxalacetat-Transaminase. In: Methoden der enzymatischen Analyse, pp. 1636–1639, Bergmeyer, H.U., ed. Verlag Chemie, Weinheim

    Google Scholar 

  • Nuernbergk, E.L. (1961) Endogener Rhythmus und CO2-Stoffwechsel bei Pflanzen mit diurnalem Säurerhythmus. Planta 56, 28–70

    Google Scholar 

  • Osmond, C.B., Allaway, W.G. (1974) Pathways of CO2 fixation during photosynthesis in Kalanchoë daigremontiana. I. Patterns of 14C fixation in the light. Aust. J. Plant Physiol. 1, 503–512

    Google Scholar 

  • Pongratz, P., Beck, E. (1978) Diurnal oscillation of amylolytic activity in spinach chloroplasts. Plant Physiol. 62, 687–689

    Google Scholar 

  • Scholander, P.F., Hammel, H.T., Hemmingsen, E.A., Bradstreet, E.D. (1964) Hydrostatic pressure and osmotic potential in leaves of mangroves and some other plants. Proc. Natl. Acad. Sci. USA 52, 119–129

    Google Scholar 

  • Warren, D.M., Wilkins, M.B. (1961) An endogenous rhythm in the rate of dark fixation of carbon dioxide in leaves of Bryophyllum fedtschenkoi. Nature (London) 191, 686–688

    Google Scholar 

  • Wilkins, M.B. (1959) An endogenous rhythm in the rate of carbon dioxide output of Bryophyllum. I. Some preliminary experiments. J. Exp. Bot. 10, 377–390

    Google Scholar 

  • Wilkins, M.B., Holowinski, A.W. (1965) The occurrence of an endogenous circadian rhythm in a plant tissue culture. Plant Physiol. 40, 907–909

    Google Scholar 

  • Wilkinson, M.J., Smith, H. (1976) Properties of phosphoenol pyruvate carboxylase from Bryophyllum fedtschenkoi leaves and fluctuations in carboxylase activity during the endogenous rhythm of carbon dioxide output. Plant Sci. Lett. 6, 319–324

    Google Scholar 

  • Winter, K. (1980a) Day/night changes in the sensitivity of phosphoenolpyruvate carboxylase to malate during Crassulacean acid metabolism. Plant Physiol. 65, 792–796

    Google Scholar 

  • Winter, K. (1980b) Carbon dioxide and water vapor exchange in the Crassulacean acid metabolism plant Kalanchoë pinnáta during a prolonged light period. Metabolic and stomatal control of carbon metabolism. Plant Physiol. 66, 917–921

    Google Scholar 

  • Winter, K. (1982) Properties of phosphoenolpyruvate carboxylase in rapidly prepared, desalted leaf extracts of the crassulacean acid metabolism plant Mesembryanthemum crystallinum L. Planta 154, 298–308

    Google Scholar 

  • Ziegler, H., Egle, K. (1965) Zur quantitativen Analyse der Chloroplastenpigmente. I. Kritische Überprüfung der spektralphotometrischen Chlorophyll-Bestimmung. Beitr. Biol. Pflanz. 41, 11–37

    Google Scholar 

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Authors and Affiliations

  1. Institut für Botanik, Technische Hochschule, Schnittspahnstrasse 3-5, D-6100, Darmstadt, Federal Republic of Germany

    Irene C. Buchanan-Bollig & J. Andrew C. Smith

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  1. Irene C. Buchanan-Bollig
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  2. J. Andrew C. Smith
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Buchanan-Bollig, I.C., Smith, J.A.C. Circadian rhythms in crassulacean acid metabolism: phase relationships between gas exchange, leaf water relations and malate metabolism in Kalanchoë daigremontiana . Planta 161, 314–319 (1984). https://doi.org/10.1007/BF00398721

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  • DOI: https://doi.org/10.1007/BF00398721

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