Summary
Chromosome deletion at the short arm of one chromosome 1 (1p32)—the most common aberration in neuroblastoma cells—was found to be combined with the generation of a homogeneously staining region at this specific site in a newly established neuroblastoma cell line (GI-LI-N) from a stage IV neuroblastoma. By in situ hybridization this homogeneously staining region was shown to contain multiple copies of the proto-oncogene N-myc. This 30-fold oncogene amplification was confirmed by Southern-blot and DNA-dot-blot analyses. In two additional cell lines from children with stage IV neuroblastoma (GI-ME-N and GI-CA-N) N-myc amplification was not detected. Chromosome 1, however, was involved in a structural rearrangement in one cell line (GI-ME-N).
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This study was supported by the Deutsche Forschungsgemeinschaft (SFB 215-A7)
Recipient of a fellowship within the Italian-German agreement on Collaborative Cancer Research in Pediatric Oncology
Supported by the Deutsche Forschungsgemeinschaft (SFB 103-C11)
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Longo, L., Christiansen, H., Christiansen, N.M. et al. N-myc amplification at chromosome band 1p32 in neuroblastoma cells as investigated by in situ hybridization. J Cancer Res Clin Oncol 114, 636–640 (1988). https://doi.org/10.1007/BF00398190
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DOI: https://doi.org/10.1007/BF00398190