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Histiocytic differentiation in benign and malignant bone tumors

  • Original Papers
  • Experimental Oncology
  • Published:
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Summary

In this study fresh frozen tissue samples of benign osseous tumors (five non-osteogenic fibromas, one fibrous dysplasia, one chondromyxoidfibroma), tumors of uncertain biological behaviour (eight cases of histiocytosis X, two giant-cell tumors), and of malignant intraosseous tumors (two malignant fibrous histiocytomas, two malignant histiocytosis, four osteosarcomas, one chondrosarcoma and two Ewing sarcomas) were studied with a panel of monoclonal antibodies reactive with monocyte/macrophages and various types of dendritic cells. In addition, tumors were further defined with a broad spectrum of antibodies against filamentous proteins and lymphocyte differentiation antigens. The specimens were stained with a triple-layer immunoalkaline phosphatase protocol. Tumors stained with these antibodies could be roughly divided into two groups. The first group comprised tumors with one predominant cell population reactive with one particular monoclonal antibody. In this group, cases of histiocytosis X were found to be consistently labelled with CD-1 antibodies. The giantcell tumors showed a very homogeneous staining with certain monocyte/macrophage antibodies (Ki-M8). Nevertheless, even in these tumors, heterogeneity was demonstrated by the occurrence of cells with monocytic differentiation in histiocytosis X and conversely by the occurrence of cells with differentiation antigens of the dendritic cell system in giant-cell tumors. An exception has to be made for the two cases of malignant histocytosis examined. These tumors were selectively labelled with antibodies against monocyte/macrophages (Ki-M8, IOM-1). The second group comprised tumors showing a high degree of heterogeneity demonstrated by the varying amounts of tumor cells reacting with the applied markers of the monocyte/macrophage and dendritic cell systems. In most cases it was difficult to ascribe labelled cells to the tumor cell population as opposed to an “innocent bystander” inflammatory cell population. This distinction was especially difficult in malignant fibrous histiocytomas underlining the current concept that these tumors are of primitive mesenchymal rather than true histiocytic origin.

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Authors and Affiliations

  1. Institute of Pathology (Head: Prof Dr. G. Seifert), University of Hamburg, Martinistrasse 52, D-2000, Hamburg 20, Federal Republic of Germany

    Andreas Meyer, Tillmann Steinmeier, Thomas Löning & Günter Delling

  2. Institut of Pathology (Head: Prof. Dr. K. Lennert), Umversity of Kiel, Hospitalstrasse 42, D-2300, Kiel, Federal Republic of Germany

    Heinz Joachim Radzun

Authors
  1. Andreas Meyer
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  2. Tillmann Steinmeier
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  3. Thomas Löning
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  4. Heinz Joachim Radzun
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  5. Günter Delling
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This study was supported by Deutsche Forschungsgemeinschaft and Hamburger Stiftung zur Förderung der Krebsbekämpfung

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Meyer, A., Steinmeier, T., Löning, T. et al. Histiocytic differentiation in benign and malignant bone tumors. J Cancer Res Clin Oncol 114, 565–574 (1988). https://doi.org/10.1007/BF00398178

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  • DOI: https://doi.org/10.1007/BF00398178

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