Abstract
The incorporation of deuterium from deuterium oxide into the free amino acids of the cotyledons of Sinapis alba L. was studied by gas chromatography-mass spectrometry and was similar, both qualitatively and quantitatively, after incubation of the seedlings in darkness or far-red light. The results support studies which show that phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) is synthesised de novo, rather than activated, in response to far-red light.
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Abbreviations
- GC-MS:
-
Gas chromatography-mass spectrometry
- PAL:
-
phenylalanine ammonia-lyase (EC 4.3.1.5)
- HFB n-propyl:
-
heptafluorobutyryl n-propyl
References
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Shaw, N.M., Parsley, K.R. & Davies, D.D. Amino-acid biosynthesis in the cotyledons of Sinapis alba L. in darkness and far-red light studied by deuterium labelling and mass spectrometry. Planta 165, 561–568 (1985). https://doi.org/10.1007/BF00398104
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DOI: https://doi.org/10.1007/BF00398104