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Hydrogen production by photosystem I of Scenedesmus: Effect of heat and salicylaldoxime on electron transport and photophosphorylation

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Summary

Photosynthesis, photoreduction, the p-benzoquinone Hill reaction, and glucose uptake by whole cells, as well as cyclic photophosphorylation (with PMS) by chloroplast particles were strongly inhibited by 10-2 M salicylaldoxime or by heating whole cells for 1–2 min at 55°. In contrast, H2 photoproduction by whole cells of mutant No. 11 and wild type Scenedesmus and PS I-mediated MR reduction by chloroplast particles were either stimulated or not significantly inhibited by these agents. H2 production by mutant No. 8 was slightly depressed by salicylaldoxime. DCMU inhibited H2 photoproduction with 10-2 M salicylaldoxime approximately 20%, indicating some contribution of electrons by endogenous organic compounds to photosystem II between the O2-evolving mechanism and the DCMU-sensitive site. We conclude that photohydrogen production by PS I of Scenedesmus does not require cyclic photophosphorylation but is due to non-cyclic electron flow from organic substrate(s) through PS I to hydrogenase where molecular H2 is released.

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Abbreviations

CI-CCP:

carbonyl cyanide m-chlorophenylhydrazone

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

DCPIP:

dichlorophenol-indophenol

MR:

methyl red

PMS:

phenazine methosulfate

PS:

photosystem

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Authors and Affiliations

  1. Institute of Molecular Biophysics, Department of Biological Science, Florida State University, Tallahassee

    Tim S. Stuart

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  1. Tim S. Stuart
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This work was supported by contract AT-(40-1)-2687 from the U.S. Atomic Energy Commission to Professor H. Gaffron.

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Stuart, T.S. Hydrogen production by photosystem I of Scenedesmus: Effect of heat and salicylaldoxime on electron transport and photophosphorylation. Planta 96, 81–92 (1971). https://doi.org/10.1007/BF00397907

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  • DOI: https://doi.org/10.1007/BF00397907

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