Summary
Sterilized ovules of a suitable age are cut in half and introduced to a culture medium kept in constant motion. Many of the embryos are washed out of the ovules and grow. The success rate compares well with more sophisticated techniques. Several plants of synthetic B. napus have been grown by this method.
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References
CaplinS. M. & StewardF. C., 1949. A technique for the controlled growth of excised plant tissues in liquid media under aseptic conditions. Nature 163: 920–921.
HakanssonA., 1956. Seed development in Brassica oleracea and B. rapa after certain reciprocal pollinations. Hereditas 42: 373–396.
Nishi, S., Kawata, J. & Toda, M., 1961. Studies on the embryo culture of cruciferous vegetables. (In Japanese, Abstract in Pl. Breed. Abstr. 32, abstract No. 2566).
PecketR. C. & SelimA. R. A. A., 1965. Embryo culture in Lathyrus. J. Exp. Bot. 16: 325–328.
RaghavanV. & TorreyJ. G., 1963. Growth and morphogenesis of globular and older embryos of Capsella in culture. Am. J. Bot. 50: 540–551.
RappaportJ., 1954. In vitro culture of plant embryos and factors controlling their growth. Bot. Rev. 20: 201–225.
RijvenA. H. G. C., 1952. In vitro studies on the embryo of Capsella bursa-pastoris. Acta Bot. Neerl. 1: 157–200.
White, P. R., 1943. Handbook of plant tissue culture. Lancaster, Pennsylvania.
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Harberd, D.J. A simple effective embryo culture technique for brassica. Euphytica 18, 425–429 (1969). https://doi.org/10.1007/BF00397792
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DOI: https://doi.org/10.1007/BF00397792