Skip to main content
Log in

A simple effective embryo culture technique for brassica

  • Published:
Euphytica Aims and scope Submit manuscript

Summary

Sterilized ovules of a suitable age are cut in half and introduced to a culture medium kept in constant motion. Many of the embryos are washed out of the ovules and grow. The success rate compares well with more sophisticated techniques. Several plants of synthetic B. napus have been grown by this method.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • CaplinS. M. & StewardF. C., 1949. A technique for the controlled growth of excised plant tissues in liquid media under aseptic conditions. Nature 163: 920–921.

    Google Scholar 

  • HakanssonA., 1956. Seed development in Brassica oleracea and B. rapa after certain reciprocal pollinations. Hereditas 42: 373–396.

    Google Scholar 

  • Nishi, S., Kawata, J. & Toda, M., 1961. Studies on the embryo culture of cruciferous vegetables. (In Japanese, Abstract in Pl. Breed. Abstr. 32, abstract No. 2566).

  • PecketR. C. & SelimA. R. A. A., 1965. Embryo culture in Lathyrus. J. Exp. Bot. 16: 325–328.

    Google Scholar 

  • RaghavanV. & TorreyJ. G., 1963. Growth and morphogenesis of globular and older embryos of Capsella in culture. Am. J. Bot. 50: 540–551.

    Google Scholar 

  • RappaportJ., 1954. In vitro culture of plant embryos and factors controlling their growth. Bot. Rev. 20: 201–225.

    Google Scholar 

  • RijvenA. H. G. C., 1952. In vitro studies on the embryo of Capsella bursa-pastoris. Acta Bot. Neerl. 1: 157–200.

    Google Scholar 

  • White, P. R., 1943. Handbook of plant tissue culture. Lancaster, Pennsylvania.

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Harberd, D.J. A simple effective embryo culture technique for brassica. Euphytica 18, 425–429 (1969). https://doi.org/10.1007/BF00397792

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00397792

Keywords

Navigation