Abstract
The ratio of RNA to DNA (RNA:DNA) was used to assess the relative growth rates of the hydrothermal vent vestimentiferans Ridgeia piscesae Jones and R. phaeophiale Jones. This biochemical indicator of growth is especially valuable when actual growth rates are difficult to measure. Tubeworms were collected from five hydrothermally active sites along the Juan de Fuca Ridge, in the Northeast Pacific Ocean in the summers of 1984 and 1986. We found significant variation in RNA:DNA among Ridgeia spp. from the five sites which was not due to size of the tubeworms or to a species-specific difference. Instead, differences in RNA:DNA were related to site of collection. Mean RNA:DNAs of 2.1 and 3.9 for R. piscesae from two sites were significantly different from each other, but not from that of tubeworms from a third site (mean=2.9). Similarly, mean RNA:DNAs of 2.3 and 4.5 for R. phaeophiale from two sites were significantly different. These patterns in RNA:DNA may reflect differences in growth rates arising from variation in environmental factors over spatial scales as small as 2 m.
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Arp, A. J., Childress, J. J., Fisher, C. R., Jr. (1984). Metabolic and blood gas transport characteristics of the hydrothermal vent bivalve Calyptogeng magnifica. Physiol. Zoöl. 57: 648–662
Buckley, L. J. (1984). RNA-DNA ratio: an index of larval fish growth in the sea. Mar. Biol. 80: 291–298
Cavanaugh, C. M., Gardiner, S. L., Jones, M. L., Jannasch, H. W., Waterbury, J. B. (1981). Prokaryotic cells in the hydrothermal vent tubeworm Riftia pachyptila Jones: possible chemoautotrophic symbionts. Science, N.Y. 213: 340–342
Davis, E. E., Currie, R. G., Riddihough, R. P., Sawyer, B. S. (1985). A new look at the Juan de Fuca Ridge: high resolution bathymetry and side-scan acoustic imagery. Geos (Ottawa) 14: 10–15
Felbeck, H. (1981). Chemoautotrophic potential of the hydrothermal vent tubeworm Riftia pachyptila Jones (Vestimentifera). Science, N.Y. 213: 336–338
Fisher, C. R., Jr., Childress, J. J. (1984). Substrate oxidation by trophosome tissue from Riftia pachyptila Jones (phylum Pogonophora). Mar. Biol. Lett. 5: 171–183
Grassle, J. F. (1985). Hydrothermal vent distribution and biology. Science, N.Y. 229: 713–717
Hessler, R. R., Smithey, W. R., Jr. (1983). The distribution and community structure of megafauna at the Galapagos rift hydrothermal vents. In: Rona P. A. et al. (ed.) Hydrothermal processes at seafloor spreading centers. Plenum Press, New York, p. 735–770
Hettmansperger, T. P. (1984). Statistical inference based on ranks. John Wiley & Sons, New York
Holm-Hansen, O. (1969). Algae: amounts of DNA and organic carbon in single cells. Science, N.Y. 163: 87–88
Jannasch, H. W., Mottl, M. (1985). Geomicrobiology of deep-sea hydrothermal vents. Science, N.Y. 229: 717–725
Johnson, K. S., Beehler, C. L., Sakamoto-Arnold, C. M., Childress, J. J. (1986). In situ measurements of chemical distributions in a deep-sea hydrothermal vent field. Science, N.Y. 231: 1139–1141
Jones, M. L. (1985). The vestimentiferans of the Eastern Pacific with comments on specimens from the Gulf of Mexico. Bull. biol. Soc. Wash. 1985 (6): 117–158
Karsten, U., Wollenberger, A. (1977). Improvements in the ethidium bromide method for direct fluorometric estimation of DNA and RNA in cell and tissue homogenates. Analyt. Biochem. 77: 464–470
Kennell, D., Magasanik, B. (1962). The relation of ribosome content to the rate of enzyme synthesis in Aerobacter aerogenes. Biochim. biophys. Acta 55: 139–151
Lehninger, A. L. (1975) Biochemistry. Worth Publishers, New York
Lilley, J., Baross, J. A., Gordon, L. I. (1983). Reduced gases and bacteria in hydrothermal fluids: the Galapagos spreading center and 21 °N East Pacific Rise. In: Rona P. A. et al. (ed.) Hydrothermal processes at seafloor spreading centers. Plenum Press, New York, p. 411–449
Lutz, R. A., Fritz, L. W., Rhoads, D. C. (1985). Molluscan growth at deep-sea hydrothermal vents. Bull. biol. Soc. Wash. 1985 (6): 119–210
Meinke, W., Goldstein, D. A., Hall, M. R. (1974). Rapid isolation of mouse DNA from cells in tissue culture. Analyt. Biochem. 58: 82–88
Regnault M., Luquet, P. (1974). Study by evolution of nucleic acid content of prepuberal growth in the shrimp Crangon vulgaris. Mar. Biol. 25: 291–298
Rhoads, D. C., Lutz, R. A., Revelas, E. C., Cerrato, R. M. (1981). Growth of bivalves at deep-sea hydrothermal vents along the Galapagos Rift. Science, N.Y. 214: 911–913
Smith, K. L., Jr. (1985). Deep-sea hydrothermal vent mussels: nutritional state and distribution at the Galapagos Rift. Ecology 66: 1067–1080
Sutcliffe, W. H., Jr. (1970). Relationship between growth rate and ribonucleic acid concentration in some invertebrates. J. Fish. Res. Bd Can. 27: 606–609
Tate, M. W., Clelland, R. C. (1957). Nonparametric and shortcut statistics in the social, biological and medical sciences. Interstate Printers & Publishers, Danville
Tunnicliffe, V., Juniper, S. K., deBurgh, M. E. (1985). The hydrothermal vent community on axial seamount, Juan de Fuca Ridge. Bull. biol. Soc. Wash. 1985 (6): 453–464
Turekian, K. K., Cochran, J. K. (1981). Growth rate of a vesicomvid clam from the Galapagos spreading center. Science, N.Y. 214: 909–911
Turekian, K. K., Cochran, J. K., Bennett, J. T. (1983). Growth rate of a vesicomyid clam from the 21 °N East Pacific Rise hydrothermal area. Nature, Lond. 303: 55–56
Tuttle, J. H. (1985). The role of sulfur-oxidizing bacteria at deep-sea hydrothermal vents. Bull. biol. Soc. Wash. 1985 (6): 335–343
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Communicated by P.C. Schroeder, Pullman
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DeBevoise, A.E., Taghon, G.L. RNA:DNA ratios of the hydrothermal-vent vestimentiferans Ridgeia piscesae and R. phaeophiale indicate variations in growth rates over small spatial scales. Mar. Biol. 97, 421–426 (1988). https://doi.org/10.1007/BF00397772
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DOI: https://doi.org/10.1007/BF00397772