Summary
Previous work has shown that loss of function mutations (designated are Ar) in areA, a positive acting regulatory gene mediating nitrogen metabolite repression in Aspergillus nidulans, lead to inability to utilise nitrogen sources other than ammonium. This work establishes the existence of a gene designated areB where mutations can suppress areAr mutations in a locus-specific manner for expression of apparently all of the genes under areA control. areB mutations are partially dominant in diploids, extremely rare and, to varying degrees, deleterious. In agreement with their ability to suppress areAr mutations, areB mutations lead to nitrogen metabolite derepressed enzyme levels. areB is located about 5 cM from creA on the left arm of linkage group I. Four of the five areB mutations selected are accompanied by translocations. In at least two of these cases the areB mutations are extremely tightly linked to and probably identical with the translocation breakpoints. These two translocations, although induced in different strains in separate experiments, apparently have almost identical breakpoints. This suggests that chromosomal rearrangements of a highly specific nature can give rise to an areB mutant phenotype.
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References
Arst HN Jr (1981) Aspects of the control of gene expression in fungi. In: Glover SW, Hopwood DA (eds) Genetics as a tool in microbiology. Society for General Microbiology Symposium 31. Cambridge University Press, Cambridge, pp 131–160
Arst HN Jr (1983) Fungal systems. In: Maclean N, Gregory SP, Flavell RA (eds.). Eukaryotic genes; their structure, activity and regulation. Butterworths, London (in press)
Arst HN Jr, Bailey CR (1977) The regulation of carbon metabolism in Aspergillus nidulans. In: Smith JE, Pateman JA (eds) Genetics and physiology of Aspergillus. Academic Press, London, pp 131–146
Arst HN Jr, Cove DJ (1969) J Bacteriol 98:1284–1293
Arst HN Jr, Cove DJ (1973) Mol Gen Genet 126:111–141
Arst HN Jr, Macdonald DW (1975) Nature (London) 254:26–31
Arst HN Jr, Scazzocchio C (1975) Nature (London) 254:31–34
Bailey C, Arst HN Jr (1975) Eur J Biochem 51:573–577
Bailey CR, Arst HN Jr, Penfold HA (1980) Genet Res 36:167–180
Bartnik E, Guzewska J, Klimczuk J, Piotrowska M, Weglenski P (1977) Regulation of arginine catabolism in Aspergillus nidulans. In: Smith JE, Pateman JA (eds) Genetic and physiology of Aspergillus. Academic Press, London, pp 243–254
Clutterbuck AJ (1974) Aspergillus nidulans In: King RC (ed) Handbook of genetics, vol 1. Plenum Press, New York, pp 447–510
Clutterbuck AJ (1981) Aspergillus News Letter 15:58–75
Cove DJ (1966) Biochim Biophys Acta 113:51–56
Cove DJ (1979) Biol Rev 54:291–327
Grove G, Marzluf GA (1981) J Biol Chem 256:463–470
Guidi-Rontani C, Danchin A, Ullmann A (1980) Proc Natl Acad Sci USA 77:5799–5801
Hynes MJ (1975) Aust J Biol Sci 28:301–313
Jones SA, Arst HN Jr, Macdonald DW (1981) Curr Genet 3:49–56
Käfer E (1977) Adv Genet 19:33–131
Klimczuk J, Weglenski P (1974) Acta Microbiol Pol A6:93–100
Lilly LJ (1965) Mutat Res 2:192–195
McCully KS, Forbes E (1965) Genet Res 6:352–359
Marzluf GA (1981) Microbiol Rev 45:437–461
Page MM (1971) Genetic and biochemical studies on the catabolism of amines and alcohols in Aspergillus nidulans. Ph.D. Thesis, University of Cambridge
Pastan I, Adhya S (1976) Bacteriol Rev 40:527–551
Paszewski A, Grabski J (1974) Mol Gen Genet 132:307–320
Paszewski A, Grabski J (1975) J Bacteriol 124:893–904
Pontecorvo G, Roper JA, Hemmons LA, Macdonald KD, Bufton AWJ (1953) Adv Genet 5:141–238
Putrament A, Guzewska J, Pieniazek D (1970) Mol Gen Genet 109:209–218
Rand KN, Arst HN Jr (1977) Mol Gen Genet 155:67–75
Rowe TC, Rusche JR, Brougham MJ, Hollomon WK (1981) J Biol Chem 256:10354–10361
Silverstone AE, Goman M, Scaife JG (1972) Mol Gen Genet 118:223–234
Sternglanz R, DiNardo S, Voelkel KA, Nishimura Y, Hirota Y, Becherer K, Zumstein L, Wang JC (1981) Proc Natl Acad Sci USA 78:2747–2751
Stunnenberg HG, Wennekes LMJ, Spierings T, van den Broek HWJ (1981) Eur J Biochem 117:121–129
Tollervey DW (1981) Aspects of nitrogen metabolic regulation in Aspergillus nidulans. Ph.D. Thesis, University of Cambridge
Tollervey DW, Arst HN Jr (1981) Current Genetics 4:63–68
Travers AA, Buckland R, Goman M, Le Grice SSG, Scaife JG (1978) Nature (London) 273:354–358
Weglenski P (1966) Genet Res 8:311–321
Winther MD, Stevens L (1981) Microbios 30:153–162
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Tollervey, D.W., Arst, H.N. Domain-wide, locus-specific suppression of nitrogen metabolite repressed mutations in Aspergillus nidulans . Curr Genet 6, 79–85 (1982). https://doi.org/10.1007/BF00397645
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DOI: https://doi.org/10.1007/BF00397645