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Fractionation and identification of Euglena gracilis cytoplasmic and chloroplastic tRNAs and mapping of tRNA genes on chloroplast DNA

Current Genetics volume 6, pages 63–69 (1982)Cite this article

Summary

The cytoplasmic and chloroplast tRNAs of Euglena gracilis Z strain were fractionated by two-dimensional gel electrophoresis and identified by aminoacylation. Purified chloroplast tRNAs, labeled in vitro with |32P|, were hybridized to endonuclease restriction fragments of chloroplast DNA, allowing the corresponding tRNA genes to be localized on the physical map of Euglena chloroplast DNA.

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Affiliations

  1. Institut de Biologie Moléculaire et Cellulaire du CNRS, Université Louis Pasteur, 15 rue Descartes, 67084, Strasbourg, France

    M. Kuntz, M. Keller, E. J. Crouse, G. Burkard & J. H. Weil

Authors
  1. M. Kuntz
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  2. M. Keller
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  3. E. J. Crouse
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  4. G. Burkard
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  5. J. H. Weil
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Kuntz, M., Keller, M., Crouse, E.J. et al. Fractionation and identification of Euglena gracilis cytoplasmic and chloroplastic tRNAs and mapping of tRNA genes on chloroplast DNA. Curr Genet 6, 63–69 (1982). https://doi.org/10.1007/BF00397643

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  • DOI: https://doi.org/10.1007/BF00397643

Key words

  • Euglena gracilis Z strain
  • tRNAs
  • Chloroplast tRNA genes
  • Hybridization