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r-DNA plasmid from Schizosaccharomyces pombe: Cloning and use in yeast transformation

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Summary

  1. 1.

    an extrachromosomal, extramitochondrial DNA circle of about 3 μm length, occurring in S. pombe, was cloned in pBR322 and a detailed restriction map of the cloned sequence constructed.

  2. 2.

    Hybridization of this DNA with the ribosomal DNA from S. cerevisiae revealed regions of homology which were mapped in the cloned S. pombe DNA.

  3. 3.

    A transformation system was developed using the URA3 gene of S. cerevisiae and the 3 μm molecule. It was shown that the r-DNA part of the hybrid plasmid promotes replication in S. pombe.

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Abbreviations

kbp:

kilo base pairs

rDNA (RNA):

ribosomal DNA (RNA)

mitDNA:

mitochondrial DNA

PMSF:

phenylmethyl-sulfonide fluoride

References

  • Anziano P, Perlman P, Lang B, Wolf K (1982) submitted for publication

  • Bach ML, Lacroute F, Botstein D (1979) Proc Natl Acad Sci USA 76:386–390

    Google Scholar 

  • Beach D, Nurse P (1981) Nature 290:140–142

    Google Scholar 

  • Clark-Walker GD, Azad AA (1980) Nucleic Acids Res 8:1009–1022

    Google Scholar 

  • Chevallier MR, Aigle M (1979) FEBS Let 108:179–180

    Google Scholar 

  • Clewell DB (1972) J Bacteriol 110:667–676

    Google Scholar 

  • Davis RW, Botstein D, Roth JR (1980) In: Advanced bacterial genetics. Cold Spring Habor Laboratory, Cold Spring Harbor

    Google Scholar 

  • Del Giudice L, Wolf K, Sassone-Corsi P, Mazza A (1979) Mol Gen Genet 172:165–169

    Google Scholar 

  • Del Giudice L (1981) Mol Gen Genet 184:465–470

    Google Scholar 

  • Erhart E, Hollenberg CP (1981) Curr Genet 3:83–89

    Google Scholar 

  • Gerbaud C, Guerineau M (1980) Curr Genet 1:219–228

    Google Scholar 

  • Hartley JL, Donelson JE (1980) Nature 286:860–864

    Google Scholar 

  • Jackson EN, Miller JH, Adams MC (1978) J Mol Biol 118:347–363

    Google Scholar 

  • Kornberg A (1980) In: DNA Replication. Freeman W. H. & Co, San Francisco

    Google Scholar 

  • Lang B, Kaudewitz F (1982) submitted for publication

  • Larionov VL, Grishin AV, Smirnov MN (1980) Gene 12:41–49

    Google Scholar 

  • Leupold U (1955) Schweiz Z Allg Path Bakteriol 15:1141–1146

    Google Scholar 

  • Miller JH (1972) In: Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor

    Google Scholar 

  • Rigby PW, Diekman M, Rhodes C, Berg P (1977) J Mol Biol 113:237–251

    Google Scholar 

  • Southern EM (1975) J Mol Biol 98:503–517

    Google Scholar 

  • Szostak JW, Wu R (1979) Plasmid 2:536–554

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Génétique, Institut National Agronomique, 16 Rue Claude Bernard, F-75231, Paris Cedex 05, France

    P. Fournier, C. Gaillardin, L. de Louvencourt & H. Heslot

  2. Institut für Genetik und Mikrobiologie der Universität Manchen, Maria-Ward-Str. 1a, D-8000, München 19, Germany

    B. F. Lang & F. Kaudewitz

Authors
  1. P. Fournier
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  2. C. Gaillardin
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  3. L. de Louvencourt
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  4. H. Heslot
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  5. B. F. Lang
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  6. F. Kaudewitz
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Fournier, P., Gaillardin, C., de Louvencourt, L. et al. r-DNA plasmid from Schizosaccharomyces pombe: Cloning and use in yeast transformation. Curr Genet 6, 31–38 (1982). https://doi.org/10.1007/BF00397639

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  • DOI: https://doi.org/10.1007/BF00397639

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