Summary
A simple, quick technique for isolating sets of a, α, a/α, a/a and α/α isogenic strains of the yeast, Saccharomyces cerevisiae is described. Isogenic a/α diploids arise in haploid populations by a rare heterothallic switch of mating type followed by mating of the switched cell with one of the other cells in the population. Sucrose density gradient centrifugation was used to select for large elliptical diploid cells in a population of smaller haploid cells, since diploid cells are larger and more oval than haploid cells. From an a/α diploid strain obtained in this manner, ala and α/α cells were isolated by selecting for mating ability using a procedure similar to marker recovery. Finally isogenic a and α haploids were simply obtained by sporulation and dissection of an a/α isogenic diploid strain.
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References
Adams J (1977) Exp Cell Res 106:267–275
Adams J, Hansche P (1974) Genetics 76:327–338
Carter B, Sudbury P (1980) Genetics 96:561–566
Conde J, Fink G (1976) Proc Natl Acad Sci USA 74:3651–3655
Davidow L, Goetsch L, Byers B (1980) Genetics 94:581–595
Fowell R (1969) Sporulation and hybridization of yeasts. In: Rose A, Harrison J (eds) The Yeasts, Academic Press Inc, London, New York, pp 461–472
Hawthorne D (1963) Genetics 48:1727–1729
Klar A (1980) Genetics 94:597–605
Laskowski V (1960) Z Naturforsch 156:495–506
Lieblová J, Beran K, Streiblová, E (1964) Folia Microbiol 9:205–213
Mortimer R, Schild D (1980) Microbiol Rev 44:519–571
Mortimer R, Hawthorne D (1969) Yeast Genetics. In: Rose AH, Harrison JS (Eds) The Yeasts, Academic Press Inc, London New York, pp 386–460
Roman H (1956) Cold Spring Harbour Symp Quant Biol 21:175–185
Schild D, Honnavara A, Mortimer RK (1981) Genetics 97:551–562
Strathern J, Newton C, Herskowitz I, Hicks J (1979) Cell 18:309–319
Thuriaux P, Nurse P, Carter B (1978) Mol Gen Genet 161:215–220
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Paquin, C., Adams, J. Isolation of Sets of a, α, a/α, a/a and α/α isogenic strains in Saccharomyces cerevisiae . Curr Genet 6, 21–24 (1982). https://doi.org/10.1007/BF00397636
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DOI: https://doi.org/10.1007/BF00397636