Abstract
Protoplasts prepared from the upper epidermis of tobacco (Nicotiana tabacum L.) leaves produce tubular structures which are highly fluorescent in the presence of Calcofluor white. The presence of Ca2+ in the incubation medium appears to be necessary for the production of these fibers. A commercial cellulase preparation destroyed these structures and inhibited their production.
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References
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Fannin, F.F., Shaw, J.G. Production of extracellular fibers by tobacco leaf epidermal protoplasts. Planta 159, 282–285 (1983). https://doi.org/10.1007/BF00397537
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DOI: https://doi.org/10.1007/BF00397537