Abstract
A genotype of Trifolium repens L. capable of sustaining high-frequency plant regeneration from long-term (24-month old) cell cultures has been selected. Numerous densely cytoplasmic meristemoids were formed in suspension cultures following the coordinate removal of 2,4-dichlorophenoxyacetic acid (2,4-D) and trichloropicolinic acid (picloram) from the medium and an increase in the NH +4 concentration. Some meristemoids arose from single cells in culture. Increasing the NH +4 concentration in the medium resulted in increased meristemoid formation and decreased the growth rate. Ammonium stimulated meristemoid formation when it was the sole source of nitrogen only if a lethal shift in the pH of the medium was prevented. Meristemoids plated on hormone-free agar medium developed directly into shoots which spontaneously formed roots.
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Abbreviations
- 2,4-D:
-
dichlorophenoxyacetic acid
- MS:
-
Murashige-Skoog (1962) medium
- NAA:
-
α-naphthaleneacetic acid
- SH:
-
Schenk-Hildebrandt (1972) medium
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White, D.W.R. Plant regeneration from long-term suspension cultures of white clover. Planta 162, 1–7 (1984). https://doi.org/10.1007/BF00397413
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DOI: https://doi.org/10.1007/BF00397413