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The function of proteases during the light-dependent transformation of etioplasts to chloroplasts in barley (Hordeum vulgare L.)

Abstract

The role of proteolysis during the light-induced rapid decrease of the NADPH: protochlorophyllide oxidoreductase in barley was studied. A proteolytic activity with a pH optimum of 4.5 was present in a plastid preparation of etiolated barley seedlings. No other proteolytic activity could be detected. The temperature optimum for the proteolysis was 50°C, and the highest specific activity was measured with hemoglobin as the substrate. In contrast to previous proposals, no evidence for the specific involvement of this protease was found during the light-induced transformation of etioplasts to chloroplasts.

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Abbreviations

SDS:

sodium dodecyl sulfate

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Correspondence to Klaus Apel.

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Dehesh, K., Apel, K. The function of proteases during the light-dependent transformation of etioplasts to chloroplasts in barley (Hordeum vulgare L.). Planta 157, 381–383 (1983). https://doi.org/10.1007/BF00397412

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Key words

  • Hordeum (plastid transformation)
  • Light and etioplast transformation
  • Protochlorophyllide oxidoreductase
  • Proteolysis