Abstract
The oxidation of carbohydrate by the pentose-phosphate pathway in the shoot apical meristem and developing leaf primordia of Dianthus chinensis was assessed by measuring the activity of glucose-6-phosphate dehydrogenase (EC 1.1.1.49). On a kg-1 dry weight h-1 basis, activity rose from 250 mmol in the apical meristem to 550 mmol in the first two leaf primordia and then declined to 350 mmol in the sixth pair of leaf primordia, and finally to 200 mmol in leaves just emerged from the shoot bud. Measurements of activity in the sixth leaf pair from the apex showed differential distribution in leaf tissues. Epidermal and mesophyll tissue had about the same activity as whole-leaf tissue, but vascular bundles had 70% greater activity. Within the vascular tissue, activity in the phloem was twice as high as in the xylem. When activity was expressed on a per-cell basis, there was a continuous increase from 20 fmol in the apex to 2 pmol in the sixth leaf pair. Activity on a per unit cell volume basis showed that cells of the apical meristem and the epidermis, mesophyll and xylem of the sixth leaf pair had similar values, about 30 amol; only the two youngest pairs of primordia and the phloem had values two or three times this amount.
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Abbreviations
- DW:
-
dry weight
- Glc6P:
-
glucose-6-phosphate
- Glc6PDH:
-
glucose-6-phosphate dehydrogenase
- GluDH:
-
glutamate dehydrogenase
- LP:
-
leaf pair
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Croxdale, J.G., Outlaw, W.H. Glucose-6-phosphate-dehydrogenase activity in the shoot apical meristem, leaf primordia, and leaf tissues of Dianthus chinensis L.. Planta 157, 289–297 (1983). https://doi.org/10.1007/BF00397399
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DOI: https://doi.org/10.1007/BF00397399