Abstract
In-vitro-grown cells of Mucuna pruriens, immobilized in calcium-alginate gels, were able to transform the precursor L-tyrosine into L-dihydroxyphenylalanine (L-DOPA). After the immobilization in alginate the plant cells released 90% of the produced L-DOPA into the medium; supplementation of the medium with calcium inhibited both the transformation of L-tyrosine into L-DOPA and the release of L-DOPA into the medium. Continuous illumination of the beads had a slight beneficial effect on the synthesis of L-DOPA. A simple production medium for the transformation of L-tyrosine into L-DOPA was designed. This medium contained only sucrose and sodium chloride as osmotic stabilizers, a low concentration of calcium chloride for stabilization of the alginate beads, and L-tyrosine as the precursor.
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Abbreviations
- DOPA:
-
dihydroxyphenylalanine
- MS-medium:
-
Murashige, Skoog-medium
References
Brain, K.R. (1976) Accumulation of L-DOPA in cultures from Mucuna pruriens. Plant Sci. Lett. 7, 157–161
Brodelius, P., Deus, B., Mosbach, K., Zenk, M.H. (1979) Immobilized plant cells for the production and transformation of natural products. FEBS Lett. 103, 93–97
Brodelius, P., Nilsson, K. (1980) Entrapment of plant cells in different matrices. FEBS Lett. 122, 312–316
Daxenbichler, M.E., Van Etten, C.H., Hallinan, E.A., Earle, F.R. (1971) Seeds as sources of L-DOPA. J. Med. Chem. 14, 463–465
Felix, H., Brodelius, P., Mosbach, K. (1981) Enzyme activities of the primary and secondary metabolism of simultaneously permeabilized and immobilized plant cells. Anal. Biochem. 116, 462–470
Griffith, T., Conn, E.E. (1973) Biosynthesis of 3,4-dihydroxyphenylalanine in Vicia faba. Phytochemistry 12, 1651–1656
Morris, P., Fowler, M.W. (1981) A new method for the production of fine plant cell suspension cultures. Plant Cell Tissue Organ Culture 1, 15–24
Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497
Oosterhuis, B., Brunt, K., Westerink, B.H.C., Doornbos, D.A. (1980) Electrochemical detector flow cell based on a rotating disk elektrode for continuous flow analysis and high performance liquid chromatography of catecholamines. Anal. Chem. 52, 203–205
Remmen, S.F.A., Ellis, B.E. (1980) DOPA synthesis in non-producer cultures of Mucuna deeringiana. Phytochemistry 19, 1421–1423
Veliky, I.A., Jones, A. (1981) Bioconversion of gitoxigenin by immobilized plant cells in a column bioreactor. Biotechnol. Lett. 3, 551–555
Widholm, J.M. (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol. 47, 189–194
Wykes, J.R., Dunnill, P., Lilly, M.D. (1971) Conversion of tyrosine to L-dihydroxyphenylalanine using immobilized tyrosinase. Nature (London) 230, 187
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Wichers, H.J., Malingré, T.M. & Huizing, H.J. The effect of some environmental factors on the production of L-DOPA by alginate-entrapped cells of Mucuna pruriens . Planta 158, 482–486 (1983). https://doi.org/10.1007/BF00397239
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DOI: https://doi.org/10.1007/BF00397239