Abstract
The somatic metaphase chromosomes of C. debile stain differentially with quinacrine and Giemsa respectively. After quinacrine staining the heterochromatic regions show fainter fluorescence than euchromatic regions. With Giemsa the heterochromatic regions are more deeply stained than the euchromatic regions. This is true also for slides which were pre-treated with trypsin or trichloroacetic acid. From these findings it may be supposed, that the heterochromatic regions are G-C rich. Furthermore, differences in protein composition are expected between hetero- and euchromatic regions. The results of differential staining of metaphase chromosomes of C. debile, including those which were published in previous papers, are summarized here.
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Literatur
Arrighi, F. E., Hsu, T. C.: Localization of heterochromatin in human chromosomes, Cytogenetics 10, 81–86 (1971).
Beermann, W.: Riesenchromosomen. Protoplasmatologia VI/D. Wien: Springer 1962.
Caspersson, T., Farber, S., Foley, G. E., Kudynowski, J., Simonsson, E., Wagh, U., Zech, L.: Chemical differentiation along metaphase chromosomes. Exp. Cell Res. 49, 219–222 (1968).
Ellision, J. R., Barr, H. J.: Quinacrine fluorescence of specific chromosome regions. Late replication and high A: T content in Samoaia leonensis. Chromosoma (Berl.) 36, 375–390 (1972).
Kato, H., Moriwaki, K.: Factors involved in the production of banded structures in mammalian chromosomes. Chromosoma (Berl.) 38, 105–120 (1972).
Pelling, C.: Ribonukleinsäure-Synthese der Riesenchromosomen. Autoradiographische Untersuchungen an Chironomus tentans. Chromosoma (Berl.) 15, 71–122 (1964).
Sandritter, W., Jobst, K., Rakow, L., Bosselmann, K.: Zur Kinetik der Feulgen-reaktion bei verlängerter Hydrolysezeit. Cytophotometrische Messungen im sichtbaren und ultravioletten Licht. Histochemie 4, 420–437 (1965).
Schuedl, W.: Analysis of the human karyotype using a reassociation technique. Chromosoma (Berl.) 34, 448–454 (1971).
Wang, H. C., Fedoroff, S.: Banding in human chromosomes treated with trypsin. Nature (Lond.) New Biol. 232, 31–32 (1972).
Weisblum, B., Haseth, P. L.: Quinacrine, a chromosome stain specific for deoxyadenylate-deoxythimidylate-rich regions in DNA. Proc. nat. Acad. Sci. (Wash.) 69, 629–632 (1972).
Yamasaki, N.: Differentielle Färbung der somatischen Metaphasechromosomen von Cypripedium debile. 1. Mitt. Chromosoma (Berl.) 7, 620–626 (1956).
Yamasaki, N.: Differentielle Färbung der Chromosomen der ersten meiotischen Metaphase von Cypripedium debile. Chromosoma (Berl.) 10, 454–460 (1959).
Yamasaki, N.: Differentielle Färbbarkeit der somatischen und meiotischen Metaphasechromosomen von Cypripedium debile nach DNase-Behandlung. Chromosoma (Berl.) 11, 479–483 (1961).
Yamasaki, N.: Differentielle Färbbarkeit der somatischen Metaphasechromosomen von Cypripedium debile durch die Methylgrün-Pyronin-Methode. Chromosoma (Berl.) 16, 411–414 (1965).
Yamasaki, N.: Karyotypanalyse an Hand des Färbungsmusters der Metaphasechromosomen von Cypripedium debile und Trillium kamtschaticum. Chromosoma (Berl.) 33, 372–381 (1971).
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Yamasaki, N. Differentielle Darstellung der Metaphasechromosomen von Cypripedium debile mit Chinacrin- und Giemsa-Färbung. Chromosoma 41, 403–412 (1973). https://doi.org/10.1007/BF00396498
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DOI: https://doi.org/10.1007/BF00396498