Skip to main content
SpringerLink
Log in

A rapid technique for producing silver-stained nucleolus organizer regions and trypsin-Giemsa bands on human chromosomes

  • Original Investigations
  • Published:
Human Genetics Aims and scope Submit manuscript

Summary

A simple and rapid technique is described whereby the nucleolus organizer regions (NORs) of human chromosomes can be differentially stained with silver. This staining is followed by trypsin-Giemsa banding on the same metaphase chromosomes. The metaphases simultaneously exhibit silverstained NORs and G bands, allowing for the unequivocal identification of all chromosomes and greatly facilitating studies involving the NOR-bearing acrocentrics.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arrighi, F. E., Hsu, T. C.: Localization of heterochromatin in human chromosomes. Cytogenet. 10, 81–86 (1971)

    Google Scholar 

  • Bloom, S. E., Goodpasture, C.: An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes. Hum. Genet. 34, 199–206 (1976)

    Google Scholar 

  • Caspersson, T., Zech, L., Modest, E. J., Foley, G. E., Wagh, U., Simmonsson, E.: DNA-binding fluorochromes for the study of the organization of the metaphase nucleus. Exp. Cell Res. 58, 141–152 (1969)

    Google Scholar 

  • Denton, T. E., Howell, W. M., Barrett, J. V.: Human nucleolar organizer chromosomes: Satellite associations. Chromosoma 55, 81–84 (1976)

    Google Scholar 

  • Goodpasture, C., Bloom, S. E.: Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma 53, 37–50 (1975)

    Google Scholar 

  • Howell, W. M., Denton, T. E., Diamond, J. R.: Differential staining of the satellite regions of human acrocentric chromosomes. Experientia 31, 260–262 (1975)

    Google Scholar 

  • Hungerford, D. A.: Leukocytes cultured form small inocula of whole blood and the preparation of metaphase chromosomes by treatment with hypotonic KC1. Stain Technol. 40, 333–338 (1965)

    Google Scholar 

  • Seabright, M.: A rapid banding technique for human chromosomes. Lancet 1971 II, 971–972

  • Sumner, A. T., Evans, H. J., Buckland, R. A.: A new technic for distinguishing between human chromosomes. Nature (New Biol.) 232, 821–822 (1971)

    Google Scholar 

  • Tantravahi, R., Miller, D. A., Miller, O. J.: Ag-staining of nucleolus organizer regions of chromosomes after Q-, C-, G-, or R-banding procedures. Cytogenet. Cell Genet. 18, 364–369 (1977)

    Google Scholar 

  • Zankl, H., Bernhardt, S.: Combined silver staining of the nucleolus organizing regions and Giemsa banding in human chromosomes. Hum. Genet. 37, 79–80 (1977)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Samford University, 35209, Birmingham, Alabama, USA

    W. Mike Howell & D. Ann Black

Authors
  1. W. Mike Howell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Ann Black
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Howell, W.M., Black, D.A. A rapid technique for producing silver-stained nucleolus organizer regions and trypsin-Giemsa bands on human chromosomes. Hum Genet 43, 53–56 (1978). https://doi.org/10.1007/BF00396478

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00396478

Keywords

Search

Navigation