Abstract
Seawater, when equilibrated with a sample of No. 2 fuel oil, becomes toxic in varying degrees to growth of representative types of microalgae, two blue-greens, a diatom, two greens, and a dinoflagellate. For a sensitive organism such as Thalassiosira pseudonana, Strain 3H, 5 ml of seawater equilibrated with fuel oil (containing 15 mg/l of organics) in 20 ml of growth medium is lethal, or roughly in the range of 40 to 400 ppb if the toxic material(s) constitute 1 to 10% water also immediately stops photosynthesis in organism 3H. For other microalgae tested e.g. 580 (a green alga) and PR-6 (a blue-green alga), similar effects on growth and photosynthesis were found, but required higher concentrations of the oil-equilibrated seawater. Water solubles from Kuwait or Southern Louisiana crude oils (when the straight crude oil was equilibrated 1:8 with seawater) were not toxic; however, specific fractions obtained by distillation did show some water-soluble toxicity. Growth experimetns in open or closed growth systems revealed that most organisms were inhibited by varying amounts of these two crude oils when in direct contact with them. Organism 580 would not grow above 5 μl of Southern Louisiana/25 ml of medium, or 10 μl of Kuwait/25 ml of medium (oil in direct contact with algae). With both the seawater equilibrated with fuel oil and the crude oils, the toxic activity is mainly localized in medium and higher boiling fractions derived from distillation cuts from these materials.
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Literature Cited
Blumer, M., M. Ehrhardt and J.H. Jones: The environmental fate of stranded crude oil. Deep Sea Res. 20, 239–259 (1973)
Gordon, D.C. and N.J. Prouse: The effects of three oils on marine phytoplankton photosynthesis. Mar. Biol. 22, 329–333 (1973)
Kauss, P., T.C. Hutchinson, C. Soto, J. Hellebust and M. Griffiths: The toxicity of crude oil and its components to freshwater algae. In: Proceedings of Joint Conference on Prevention and Control of Oil Spills, Washington, D.C., pp 703–714. Washington, D.C.: American Petroleum Institute 1973
Myers, J.: The culture of algae for physiological research. In: The culturing of algae, pp 45–51. Yellow Springs, Ohio: C.F. Kettering Foundation 1950
Nuzzi, R.: Effects of water soluble extracts of oil on phytoplankton. In: Proceedings of Joint conference on Prevention and Control of Oil Spills, Washington, D.C., pp 809–814. Washington, D.C.: American Petroleum Institute 1973
Perry, J.J. and C.E. Cerniglia: Studies on the degradation of petroleum by filamentous fungi. Center for Wetlands Resources, LSU-SG-73-01, pp 89–94 (1973)
Provasoli, L., J.J. McLaughlin and M.R. Droop: The development of artificial media for marine algae. Arch. Mikrobiol. 25, 392–428 (1957)
Rabinowitch, E. and Govindjee: Photosynthesis, 273 pp. New York: J. Wiley and Sons, Inc. 1969
Strand, J.A., W.L. Templeton, J.A. Lichatowich and C.W. Apts: Development of toxicity test procedures for marine phytoplankton. In: Proceedings of Joint Conference on Prevention and Control of Oil Spills, Washington, D.C., pp 279–286. New York: American Petroleum Institute 1971
Van Baalen, C.: Studies on marine blue-green algae. Botanica mar. 4, 129–139 (1962)
—: Effects of ultraviolet light on a coccoid blue-green alga: survival, photosynthesis, and photoreactivation. Pl. Physiol., Lancaster 43, 1689–1695 (1968)
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Communicated by O. Kinne, Hamburg
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Pulich, W.M., Winters, K. & Van Baalen, C. The effects of a no. 2 fuel oil and two crude oils on the growth and photosynthesis of microalgae. Mar. Biol. 28, 87–94 (1974). https://doi.org/10.1007/BF00396299
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DOI: https://doi.org/10.1007/BF00396299