Summary
A systematic search for suppressors of mutations which cause a deficiency in the splicing of mitochondrial RNA has been undertaken. These splicing mutations were localized in the mRNA-maturase coding sequence of the second intron of the cob-boxgene, i.e. in the box3locus. A total of 953 revertants (mostly spontaneous in origin) were isolated and their genetic nature (nuclear vs. mitochondrial) and phenotype characterized.
Most revertants were mitochondrially determined and displayed a wild-type phenotype. A mitochondrial suppressor unlinked with the box3 −target mutation was uncovered among the revertants displaying a pseudo-wild phenotype: out of 26 revertants analyzed, derived from 7 different box3− mutants only one such suppressor mutation mim3-1 was found. It was localized by rho− deletion mapping in the region between the oxi2 and oxi3 gene, within (or in the vicinity) the gene specifying the 15S ribosomal RNA.
Nuclear suppressors were isolated from seven different box3 −mutants. All were recessive and had a pseudo-wild phenotype. Three such suppressors nam3-1, nam3-2 and nam3-3 were investigated more extensively. Tetrad analysis has shown that they are alleles of the same nuclear locus NAM3 and mitotic analysis has shown that they do not segregate mitotically.
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References
Bolotin-Fukuhara M (1979) Mol Gen Genet 177:39–46
Borst P, Grivell LA (1978) Cell 15:705–723
Coruzzi G, Tzagoloff A (1979) J Biol Chem 254:9324–9330
Dujardin G, Pajot P, Groudinsky O, Slonimski PP (1980a) Mol Gen Genet 179:469–482
Dujardin G, Groudinsky O, Kruszewska A, Pajot P, Slonimski PP (1980b) In: Saccone C, Kroon AM (eds) The organization and expression of the mitochondrial genome. North-Holland, Amsterdam, pp 157–160
Dujardin G, Jacq C, Slonimski PP (1982) Nature 298:628–632
Dujon B (1981) In: Strathern J, Jones E, Broach JR (eds) The molecular biology of the yeast Saccharomyces cerevisiae. Cold Spring Harbor, New York, pp 505–635
Fox TD, Staempfli S (1982) Proc Natl Acad Sci USA 79:1583–1587
Gargouri A, Lazowska J, Slonimski PP (1983) In: Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1983. Nucleo-mitochondrial interactions. Walter de Gruyter, Berlin, pp 259–268
Groudinsky O, Dujardin G, Slonimski PP (1981) Mol Gen Genet 184:493–503
Handwerker A, Schweyen RJ, Wolf K, Kaudewitz F (1973) J Bacteriol 113:1307–1310
Kotylak Z, Slonimski PP (1977) In: Bandlow W, Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1977. Genetics and biogenesis of mitochondria. Walter de Gruyter, Berlin, pp 83–89
Kruszewska A (1982) In: Attardi G, Borst P, Slonimski PP (eds) Mitochondrial genes, Monograph 12. Cold Spring Harbor, New York, pp 323–326
Kruszewska A, Szczesniak B (1980) Genet Res Cam 35:225–229
Kruszewska A, Szczesniak B, Claisse M (1980) Curr Genet 2:45–51
Lamouroux A (1979) Thèse de Doctorat de 3ème Cycle de Génétique. Paris XI
Lazowska J, Jacq C, Slonimski PP (1980) Cell 22:333–348
Li M, Tzagoloff A, Underbrink-Lyon K, Martin NC (1982) J Biol Chem 257:5921–5928
Rödel G, Holl J, Schmelzer C, Schmidt C, Schweyen RJ, Weiss-Brummer B, Kaudewitz F (1983) In: Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1983. Nucleo-mitochondrial interactions. Walter de Gruyter, Berlin, pp 191–201
Schweyen RJ, Steyrer U, Kaudewitz F, Dujon B, Slonimski PP (1976) Mol Gen Genet 146:117–132
Sor F, Faye G (1979) Mol Gen Genet 177:47–56
Wesolowski M, Fukuhara H (1979) Mol Gen Genet 170:261–275
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Kruszewska, A., Slonimski, P.P. Mitochondrial and nuclear mitoribosomal suppressors that enable misreading of ochre codons in yeast mitochondria. Curr Genet 9, 1–10 (1984). https://doi.org/10.1007/BF00396198
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DOI: https://doi.org/10.1007/BF00396198