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Planta

, Volume 164, Issue 4, pp 473–479 | Cite as

Changes in dye coupling of stomatal cells of Allium and Commelina demonstrated by microinjection of Lucifer yellow

  • B. A. Palevitz
  • P. K. Hepler
Article

Abstract

Lucifer yellow has been microinjected into stomatal cells of Allium cepa L. epidermal slices and Commelina communis L. epidermal peels and the symplastic spread of dye to neighboring cells monitored by fluorescence microscopy. Dye does not move out of injected mature guard cells, nor does it spread into the guard cells when adjacent epidermal or subsidiary cells are injected. Dye does spread from injected subsidiary cells to other subsidiary cells. These results are consistent with the reported absence of plasmodesmata in the walls of mature guard cells. Microinjection was also used to ascertain when dye coupling ceases during stomatal differentiation in Allium. Dye rapidly moves into and out of guard mother cells and young guard cells. Hovewer, dye movement ceases midway through development as the guard cells begin to swell but well before a pore first opens. Since plasmodesmata are still present at this stage, the loss of symplastic transport may result from changes in these structures well in advance of their actual disappearance from the guard cell wall.

Key words

Allium (stomatal cells) Commelina Dye coupling Guard cell Lucifer yellow Plasmodesma 

Abbreviations

DIC

differential interference contrast

GMC

guard mother cell

LY

Lucifer yellow

Pd

plasmodesmata

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • B. A. Palevitz
    • 1
  • P. K. Hepler
    • 2
    • 3
  1. 1.Botany DepartmentUniversity of GeorgiaAthens
  2. 2.Botany DepartmentUniversity of MassachusettsAmherst
  3. 3.Marine Biological LaboratoryWoods HoleUSA

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