Summary
In the amphibian Pleurodeles waltlii, lamininrelated polypeptides (amphibian-LN) are present in the extracellular matrix underlying the blastocoel roof of gastrulating embryos. Immunoprecipitation with affinity-purified anti-laminin antibodies demonstrated that amphibian-LN is synthesized in oocytes (from stage III onward), eggs and throughout early development. At the late blastula stage, when experiments were carried out with animal and vegetal halves, there were no regional differences in the pattern of amphibian-LN synthesis. The results obtained with transcription inhibitors suggest that throughout pregastrula stages, amphibian-LN is a translation product of stored maternal mRNA. Finally, having compared amphibian-LN and fibronectin synthesis, it is concluded that both extracellular glycoproteins have a common pattern of synthesis.
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References
Barth LG, Barth LJ (1959) Differentiation of cells of the Rana pipiens gastrula in unconditioned medium. J Embryol Exp Morphol 7:210–222
Bonnanfant-Jaïs ML, Mentré P (1983) Study of oogenesis in the newt Pleurodeles waltlii Michah I. Ultrastructural study of different stages of oocyte development. J Submicrosc Cytol 15:453–478
Boucaut JC, Darribère T (1983) Fibronectin in early amphibian embryos. Migrating mesodermal cells contact fibronectin established prior to gastrulation. Cell Tissue Res 234:135–145
Boucaut JC, Darribère T, Boulekbache H, Thiery JP (1984) Prevention of gastrulation but not neurulation by antibodies to fibronectin in amphibian embryos. Nature (Lond) 307:364–367
Boucaut JC, Darribère T, Shi De Li, Boulekbache H, Yamada KM, Thiery JP (1985) Evidence for the role of fibronectin in amphibian gastrulation. J Embryol Exp Morphol [Suppl] 89:211–227
Chamberlain JP (1979) Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem 98:132–135
Cooper AR, McQueen HA (1983) Subunits of laminin are differentially synthesized in mouse eggs and early embryos. Dev Biol 96:467–471
Cooper AR, Kurkinen M, Taylor A, Hogan BLM (1981) Studies of the biosynthesis of laminin by murine parietal endoderm cells. Eur J Biochem 119:189–197
Critchley DR, England MA, Wakely J, Hynes RO (1979) Distribution of fibronectin in the ectoderm of gastrulating chick embryos. Nature 280:498–500
Darribère T, Boucher D, Lacroix JC, Boucaut JC (1984) Fibronectin synthesis during oogenesis and early development of the amphibian Pleurodeles waltlii. Cell Differ 14:171–177
Darribère T, Riou JF, De Li Shi, Delarue M, Boucaut JC (1986) Synthesis and distribution of laminin-related polypeptides in early amphibian embryos. Cell Tissue Res 246:45–51
Davis GE, Manthorpe M, Engvall E, Varon S (1985) Isolation and characterization of rat schwannoma neurite-promoting factor: evidence that the factor contains laminin. J Neurosci 5:2662–2671
Denis H (1966) Activité des gènes au cours du développement embryonnaire. Desoer ed. Liège
Ekblom P, Vestweber D, Kemler R (1986) Cell-matrix interactions and cell adhesion during development. Ann Rev Cell Biol 2:27–47
Engel J, Odermatt E, Engel A, Madri JA, Furthmayr H, Rohde H, Timpl R (1981) Shapes, domain organization and flexibility of fibronectin and laminin, two multifunctional proteins of the extracellular matrix. J Mol Biol 150:97–120
Gallien L, Durocher M (1957) Table chronologique développement chez Pleurodeles waltlii Michah. Bull Biol France Belgique 91:97–114
Hynes RO, Yamada KM (1982) Fibronectins. Multifunctional modular glycoproteins. J Cell Biol 95:369–377
Kleinman HK, Cannon FB, Lauri GW, Kassel JR, Aumaillay M, Terranova VP, Martin GR, Dubois-Dalq M (1985). Biological activities of laminin. J Cell Biochem 27:317–326
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–689
Lander AD, Fujii DK, Reichardt LF (1985) Purification of a factor that promotes neurite outgrowth: isolation of laminin and associated molecules. J Cell Biol 101:898–913
Lee G, Hynes RO, Kirschner M (1984) Temporal and spatial regulation of fibronectin in early Xenopus development. Cell 36:729–740
Leivo I, Vaheri A, Timpl R, Wartiovaara J (1980) Appearance and distribution of collagens and laminin in the early mouse embryo. Dev Biol 76:100–114
Liesi P (1985) Do neurons in vertebrate CNS migrate on laminin? EMBO J 5:1163–1170
Macdonald GC, Furcht LT, Brand KG (1983) Fibronectin in foreign body-induced sarcomas and preneoplastic cells. P Soc Exp Med 172:89–92
Nakatsuji N, Johnson KE (1984) Experimental manipulation of a contact guidance system in amphibian gastrulation by mechanical tension. Nature 307:453–455
Nakatsuji N, Gould AC, Johnson KE (1982) Movement and guidance of migrating mesodermal cells in Ambystoma maculatum gastrulae. J Cell Sci 56:207–222
Nakatsuji N, Hashimoto K, Hayashi M (1985) Laminin fibrils in newt gastrulae visualized by the immunofluorescent staining. Dev Growth Differ 27:639–643
Newport J, Kirschner M (1982) A major developmental transition in early Xenopus embryos. I. Characterisation and timing of cellular changes at the midblastula stage. Cell 30:675–686
Olwin BB, Hall ZW (1985) Developmental regulation of laminin accumulation in the extracellular matrix of a mouse muscle cell line. Dev Biol 112:359–367
Rogers SL, Letourneau PC, Palm SL, Mc Carthy J, Furcht LT (1983) Neurite extension by peripheral and central nervous system neurons in response to substratum-bound fibronectin and laminin. Dev Biol 98:212–220
Ruoslahti E, Pierschbacher MD (1986) Arg-Gly-Asp. A versatile cell recognition signal. Cell 44:517–518
Smith RC (1986) Protein synthesis and messenger RNA levels along the animal-vegetal axis during early Xenopus development. J Embryol Exp Morphol 95:15–35
Spiegel E, Burger M, Spiegel M (1980) Fibronectin in the developing sea urchin embryo. J Cell Biol 87:309–313
Steinberg MS (1957) In: Ebert Y (ed) Carnegie Inst. Washington Year Book 56
Timpl R, Rohde H, Gehron Robey P, Rennard SI, Foidart JP, Martin GR (1979) Laminin. A glycoprotein from basement membranes. J Biol Chem 254:8922–8927
Yamada KM (1983) Cell surface interactions with extracellular materials. Ann Rev Biochem 52:761–799
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Riou, J.F., Darribére, T., De Shi, L. et al. Synthesis of laminin-related polypeptides in oocytes, eggs and early embryos of the amphibian Pleurodeles waltlii . Roux's Arch Dev Biol 196, 328–332 (1987). https://doi.org/10.1007/BF00395957
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DOI: https://doi.org/10.1007/BF00395957