Summary
The effect of vitamins A, C and E, butylated hydroxytoluene (BHT) and glutathione (GSH) on gastric carcinogenesis induced by N-methyl-N-nitro-N-nitrosoguanidine (MNNG) was investigated. Male and female BD-VI rats 2–3 months old received a single oral application of MNNG dissolved in corn oil. The male rats were divided into four groups: Group-I: MNNG 250 mg/kg by intubation; Group-II: MNNG+ vitamin C daily in the drinking water (400 mg/l); Group-III: MNNG+vitamin C (400 mg/1) +100 g of milk broth (for each of 10 rats) containing vitamin A (40 000 IU), vitamin E (0.5 g) and BHT (0.1 g) three times a week. The treatment with antioxidants started 7 days before the MNNG administration and continued until the end of experiment. Group-IV rats received MNNG+oxyferriscorbone, i.p. as a single dose of 1.0 mg/kg, daily during the week before and the week after MNNG exposure and than 3 times a week till the end of the experiment. Female rats were divided into two groups: Group-I: MNNG 333 mg/kg by intubation; Group-II: MNNG +GSH orally at a dose of 100 mg/rat 1 h before and 5, 24, 48, and 72 h after MNNG intubation.
The incidence of gastric tumors after 15 months of treatment was as follows: male rats, 82.4% in Group-I, 40.0% in Group-II, 40.7% in Group-III, and 50.0% in Group-IV; female rats; 72.7% in Group-I, and 36.0% in Group-II.
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References
Ahluwalia MB, Rotstein J, Tatematsu M, Roomi MW, Farber E (1983) Failure of glutathione to prevent liver cancer development in rats initiated with diethylnitrosamine in the resistant hepatocyte model. Carcinogenesis, 4:119–121
Balansky RM, Blagoeva PM, Mirtcheva Z (1983) The influence of selenium and caffeine on chemical carcinogenesis in rats, mutagenesis in bacteria and unscheduled DNA synthesis in human lymphocytes. Biol Trace Element Res 5:331–343
Blagoeva PM, Balanski RM (1981) Inhibition of DENA-liver carcinogenesis in rats with sodium oxyferriscorbone. Comptes rendus de l'Acad bulgare des Sci 34, 5:711–712
Cook JR, Huang DP, Burkhardt AL, Goldman ME, Carroll M, Bresnick E, Chiu JF (1984) Assessment of the anti-tumor potential of glutathione. Cancer Lett 21:277–283
Edgar JA (1974) Ascorbic acid and biological alkylating agents. Nature 248:136–137
Fukushima S, Tatematsu M, Takahashi M (1974) Combined effect of various surfactants on gastric carcinogenesis in rats treated with N-methyl-N-nitro-N-nitrosoguanidine. Gann 65:371–376
Hirono I, Shibuya C (1972) Induction of stomach cancer by a single dose of N-methyl-N-nitro-N-nitrosoguanidine through a stomach tube. In “Topics in chemical carcinogenesis”, proceedings 2nd International Symposium Princess Takamatsu Cancer Res Fund Tokyo, pp 121–131
Ito N, Hagiwara A, Shibata M, Ogiso T, Fukushima S (1982) Induction of squamous cell carcinoma in the forestomach of F344 rats treated with butylated hydroxyanisole. Gann, 73:332–335
Kawasaki H, Morishige F, Tanaka H, Kimoto E (1982) Influence of oral supplementation of ascorbate upon the induction of N-methyl-N-nitro-N-nitrosoguanidine. Cancer Lett 16:57–63
Kobori O, Shimizu T, Maeda M, Atomi Y, Watanabe J, Shoji M, Morioka Y (1984) Enhancing effect of bile and bile acid on stomach tumorigenesis induced by N-methyl-N-nitro-N-nitrosoguanidine in Wistar rats. J Natl Cancer Inst 73:853–861
Mirvish SS, Wallcave L, Eagen M, Shubik P (1972) Ascorbate-nitrite reaction: possible means of blocking the formation of carcinogenic N-nitroso compounds. Sicence 177:65–68
Neal GE, Legg RF (1983) The ineffectiveness of reduced glutathione in preventing the development of liver tumors from aflatoxin-induced pre-neoplastic liver lesions. Cancer Lett 21:83–87
Nera EA, Lok E, Iverson F, Ormsby E, Karpinski KF, Clayson DB (1984) Short-term pathological and proliferative effects of butylated hydroxyanisole and other phenolic antioxidants in the forestomach of Fisher 344 rats. Toxicology 32:197–213
Novi AM (1981) Regression of aflatoxin B1-induced hepato-cellular carcinoma by reduced glutathione. Science 212:541–542
Robert A, Eberle D, Kaplowitz N (1984) Role of glutathione in gastric mucosal cytoprotection. Am J Physiol 247:G296-G304
Rosin MP, Stich HF (1979) Assessment of the use of the Salmonella mutagenesis assay to determine the influence of antioxidants on carcinogen-induced mutagenesis. Int J Cancer 23:722–727
Szabo S, Trier JS, Frankel PW (1981) Sulfhydryl compounds may mediate gastric cytoprotection. Science 214:200–202
Tahara E, Haizuka S (1975) Effec of gastro-enteropancreatic endocrine hormones on the histogenesis of gastric cancer in rats induced by N-methyl-N-nitro-N-nitrosoguanidine, with special reference to development of scirrhous gastric cancer. Gann 66:421–426
Tatematsu M, Takahashi M, Fukushima S, Hananouchi M, Shirai T (1975) Effects in rats of sodium chloride on experimental gastric cancers induced by N-methyl-N-nitro-N-nitrosoguanidine or 4-nitro-quinoline-1-oxide. J Natl Cancer Inst 55:101–106
Wattenberg LW (1972) Inhibition of carcinogenesis and toxic effects of polycyclic hydrocarbons by phenolic antioxidants and ethoxyquin. J Natl Cancer Inst 48:1425–1430
Wattenberg LW (1978) Inhibition of chemical carcinogenesis Adv Cancer Res 26:197–226
Wynder EL, Reddy BS, McCoy GD, Weisburger JH, Williams G (1976) Diet and gastrointestinal cancer. Clin Gastroenterol 5:463–482
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Balansky, R.M., Blagoeva, P.M., Mircheva, Z.I. et al. The effect of antioxidants on MNNG-induced stomach carcinogenesis in rats. J Cancer Res Clin Oncol 112, 272–275 (1986). https://doi.org/10.1007/BF00395922
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DOI: https://doi.org/10.1007/BF00395922