Summary
The effect of β-cyclodextrin-benzaldehyde (CDBA) on experimental pulmonary metastasis in C3H/He mice was examined. In an in vitro assay, the growth of RCT(+) cells was inhibited by 1200 μg/ml CDBA using unrenewed media, and by 600 μg/ml CDBA in that using daily renewed media. When mice were treated daily with CDBA, 3 weeks later the number of lung nodules developing after i.v. injection of 1×106 RCT(+) cells was significantly decreased in a dose-dependent manner, i.e., 73.8%, 85.6%, and 95.7% inhibition was observed following 0.5, 5, and 25 mg CDBA/mouse per day p.o. administration, respectively. The same mice showed almost as much natural killer (NK) activity as normal mice. Therefore, experiments were designed to evaluate the effect of CDBA on the NK activity of tumor-free mice whose immunity had been suppressed by 5-fluorouracil (5FU). Injections of 5FU only suppressed this activity to about 50% of normal mice, but the combined treatment with CDBA negated the suppressive effect of 5FU on NK activity. The results suggested that the inhibition of experimental pulmonary metastasis might be induced by the possible combined effects of CDBA; that is, the direct inhibition of tumors and the augmentation of NK cell activity.
Similar content being viewed by others
References
Cikes M, Firber S Jr, Klein G (1973) Progressive loss of H-2 antigens with concomitant increase of cell-surface antigen(s) determined by molony leukemia virus in cultured murine lymphomas. J Natl Cancer Inst 50:347–362
Hanna N (1982) Inhibition of experimental tumor metastasis by selective activation of natural killer cells. Cancer Res 42:1337–1342
Hanna N, Fidler IJ (1980) Role of natural killer cells in the destruction of circulating tumor emboli. J Natl Cancer Inst 65:801–809
Ishida A, Miwa N, Mizuno S (1983) Differential enhancement of cytotoxicity by combination of the carcinostatic agent benzaldehyde and hyperthermia in simian virus 40-transformed and normal cell lines. Cancer Res 43:4216–4220
Kochi M, Takeuchi S, Mizutani T, Mochizuki K, Matsumoto Y, Saito Y (1980) Antitumor activity of benzaldehyde. Cancer Treat Rep 64:21–23
Miyakawa T, Zundel JL, Sakaguchi K (1979) Selective inhibitory effect of benzaldehyde on the growth of simian virus 40-transformed cells. Biochem Biophys Res Common 87:1024–1030
Nambata T, Terada N, Mizutani T, Takeuchi S, (1980) Effects of benzaldehyde on mouse embryo cells in vitro. Gann 71:572–573
Nambata T, Terada N, Takeuchi S (1981) Effect of benzaldehyde on a malignant phenotype of transformed rat liver cells. Agric Biol Chem 45:2815–2821
Nambata T, Terada N, Mizutani T, Takeuchi S (1982) Characteristics of C3H/He mouse embryo cell lines established by culture with or without benzaldehyde. Gann 73:592–599
Pettersen EO, Nome O, Rønning ØW, Oftebro R (1983a) Effect of benzaldehyde on survival and cell-cycle kinetics of human cells cultivated in vitro. Eur J Cancer Clin Oncol 19:507–514
Pettersen EO, Rønning ØW, Nome O, Oftebro R (1983b) Effects of benzaldehyde on protein metabolism of human cells cultivated in vitro. Eur J Cancer Clin Oncol 19:935–940
Pettersen EO, Dornish JM, Rønning ØW (1985) 4,6-benzylidene-d-glucose, a benzaldehyde derivative that inhibits protein synthesis but not mitosis of NHIK 3025 cells. Cancer Res 45:2085–2091
Taetle R, Howell SB (1983) Preclinical re-evaluation of benzaldehyde as a chemotherapeutic agent. Cancer Treat Rep 67:561–566
Takeuchi S, Kochi M, Sakaguchi K, Mizutani T (1978) Benzaldehyde as a carcinostatic principle in figs. Agric Biol Chem 42:1449–1451
Watanuki M, Sakaguchi K (1980) Selective inhibition by benzaldehyde of the uptake of nucleosides and sugar into simian virus 40-transformed cells. Cancer Res 40:2574–2579
Zundel JL, Miyakawa T, Sakaguchi K (1978) Derivatives and analogues of benzaldehyde selectively cytotoxic to SV40-transformed cells. Agric Biol Chem 42:2191–2193
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ochiai, H., Niwayama, S. & Masuyama, K. Inhibition of experimental pulmonary metastasis in mice by β-cyclodextrin-benzaldehyde. J Cancer Res Clin Oncol 112, 216–220 (1986). https://doi.org/10.1007/BF00395915
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00395915