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Complexity, polymorphism, and connectivity of mouse V k gene families

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Abstract

To define the polymorphism and extent of the mouse immunoglobulin kappa (Igk) gene complex, we have analyzed restriction-enzyme digested genomic DNA from 33 inbred strains of mice with labeled DNA probes corresponding to 16 V x protein groups (1 of them previously undescribed) and the J k/C K region (V, variable; J, joining; C, constant). These probes detected between 1 and 25 distinct restriction enzyme fragments (REF) that appeared in up to eight polymorphic patterns, thus defining eight mouse Jgk haplotypes. The investigated portion of the V A repertoire was estimated to encompass between 60 and 120 discernable V k gene-containing REFs. In contrast to mouse V H gene families, several V k gene families defined by these probes appeared to overlap. This observation has implications for V k gene analyses by nucleic acid hybridization and raises the possibility that the V A gene complex is a continuum of related sequences.

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Abbreviations

C:

constant

Ig:

immunoglobulin

J:

joining

REF:

restriction enzyme fragment

RFLP:

restriction fragment length polymorphism

V:

variable

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Authors and Affiliations

  1. Department of Immunology, Research Institut of Scripps Clinic, 92037, La Jolla, CA, USA

    Reinhard Koflerz, Michel A. Duchosal & Frank J. Dixon

  2. Institute for General and Experimental Pathology, University of Innsbruck, A-6020, Austria

    Reinhard Koflerz

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  1. Reinhard Koflerz
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  2. Michel A. Duchosal
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  3. Frank J. Dixon
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Koflerz, R., Duchosal, M.A. & Dixon, F.J. Complexity, polymorphism, and connectivity of mouse V k gene families. Immunogenetics 29, 65–74 (1989). https://doi.org/10.1007/BF00395853

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  • DOI: https://doi.org/10.1007/BF00395853

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