Abstract
Experiments conducted in vitro using the electrophoretic mobility shift assay have shown that a single region of the extensin gene of carrot (Daucus carota L.) interacts with a protein factor designated Extensin Gene Binding Factor-1 (EGBF-1) present in nuclear extracts obtained from carrot roots. This interaction is sequence-specific as judged by the failure of other plant gene sequences to compete with the extensin gene for EGBF-1 binding. The EGBF-1 activity is organspecific, not being expressed in nuclear extracts obtained from carrot leaves or stems. Both ethylene treatment and wounding of roots are shown to have a controlling influence on the expression of EGBF-1 activity in nuclear extracts. These results demonstrate that at least three distinct signals: ethylene treatment, wounding, and development, are important in determining the activity of EGBF-1 in nuclear extracts, and indicate a role for EGBF-1 in stress-related signal transduction and the regulation of extensin-gene expression.
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Abbreviations
- bp:
-
base pair(s)
- EGBF:
-
extensin-gene binding factor
- EMSA:
-
electrophoretic mobility shift assay
- HRGP:
-
hydroxyproline-rich glycoprotein
- kb:
-
kilobase
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Holdsworth, M.J., Laties, G.G. Site-specific binding of a nuclear factor to the carrot extensin gene is influenced by both ethylene and wounding. Planta 179, 17–23 (1989). https://doi.org/10.1007/BF00395766
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DOI: https://doi.org/10.1007/BF00395766