Summary
Cell kinetic studies on the effect of vincristine (VCR) on cells in different cell-cycle phases were carried out using single- and double-labeling methods with 3H- and 14C-thymidine. Studies on mouse L 1210 and JB-1 ascites tumor cells, mouse jejunal crypt cells as well as on HeLa cells have shown that VCR affects cells not only during or prior to mitosis, but also cells that are in S phase at the time of VCR application. These cells are arrested during the next mitosis. Cells arrested in mitosis even with small doses of VCR subsequently become necrotic. Thus, it has been shown experimentally that an in vivo synchronization of tumor cells cannot be achieved with VCR and, therefore, the effect of a so-called synchronization therapy with VCR is not due to synchronization of tumor cells.
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References
Alabaster O, Cassidy M (1978) Flow microfluorometric analysis of P 388 murine leukemia after administration of vincristine and maytansine. In Vivo. J Natl Cancer Inst 60:649–652
Badaracco G, Morelli M, Nervi C, Starace G (1979) Cytokinetic effects of continuous infusion of vincristine in head and neck carcinoma. Eur J Cancer 15:17–26
Bartsch WH (1976) Castistic report in timing of cytostable drugs by synchronization to tumor cells. Panminerva Med 18:89–93
Basler U (1979) Experimentelle Untersuchungen zur Zellzyklusphasenspezifischen Wirkung von Vincristin unter in vivo Bedingungen. Inaugural Dissertation Würzburg
Braunschweiger PG, Schiffer LM (1978) Cell kinetics after vincristine treatment of C3H/He spontaneous mammary tumors: Implications for therapy. J Natl Cancer Inst 60:1043–1048
Camplejohn RS (1980) A critical review of the use of vincristine (VCR) as a tumour cell synchronizing agent in cancer therapy. Cell Tissue Kinet 13:327–335
Camplejohn RS, Schultze B, Maurer W (1980) An in vivo double labelling study of the subsequent fate of cells arrested in metaphase by vincristine in the JB-1 mouse ascites tomour. Cell Tissue Kinet 13:239–250
Dettmer J (1979) Zur Frage einer in vivo-Synchronisation von Ehrlich-Ascites-Tumorzellen mit Vincristin. Inaugural Dissertation Würzburg
Gross R, Klein HO (1978) Neuere Entwicklungen auf dem Gebiet der zytostatischen Kombinationschemotherapie. Dtsch Ärztebl 38:2121–2128
Hartenstein R, Ehrhart H, Hoffmann K (1973) Zellkinetische Unterschungen beim Ehrlich-Ascites-Tumor unter Vincristin. Z Krebsforsch 79:213–220
Hartwich G, Fuchs HF, Neidhardt B (1972) Zytostatische Behandlung teilsynchronisierter Tumoren. Fortschr Med 90:46–49
Hartwich G, Tschätsch W, Lutz H (1973) Zytostatische Kombinationsbehandlung mit Vincristinsulfat und Ifosfamid. Dtsch Med Wschr 98:1203–1207
Jellinghaus W, Maidhof R, Schultze B, Maurer W (1975) Experimentelle Untersuchungen und zellkinetische Berechnungen zur Frage der Synchronisation mit Vincristin in vivo (Mäuseleukämie L 1210, Krypten-Epithelien der Maus). Z Krebsforsch 84:161–176
Jellinghaus W, Schultze B, Maurer W (1977) The effect of vincristine on mouse jejunal crypt cells of differing cell age: double labelling autoradiographic studies using 3H-and 14C-TdR. Cell Tissue Kinet 10:147–156
Klein HO, Lennartz KJ, Habicht W, Eder M, Gross R (1970) Synchronisation von Ehrlich-Ascites-Tumorzellen und ihre Bedeutung bei der Anwendung eines alkylierenden Cytostaticum. Klin Wschr 48:1001–1005
Klein HO, Lennartz KJ, Gross R, Eder M, Fischer R (1972) In-vivo-und In-vitro-Untersuchungen zur Zellkinetic und Synchronisation menschlicher Tumorzellen. Dtsch Med Wschr 97:1273–1282
Klein HO, Lennartz KJ, Gross R (1973) Pharmakotogische Synchronisation von Tumorzellen und ihre Bedeutung für die cytostatische Therapie. In: G. Wüst (Hrsg) Aktuelle Probleme der Therapie maligner Tumoren. Thieme Verlag, Stuttgart (pp 270–274)
Klein HO, Lennartz KJ (1974) Chemotherapy after synchronization of tumor cells. Semin Hematol 11:203–227
Klein HO, Gerecke D, Borberg H, Gross R, Hoefer-Janker H, Scheef W, Diehl V, Lohmann E, Adler D, Butter E, Löhning A, Brock N, Burkert H (1975) Ergebnisse der Synchronisationstherapie mit VCR und Cyclophosphamid bei Lymphogranulomatose, Retikulumzell-und Lymphsarkom. Dtsch med Wschr 100:1719–1726
Klein HO, Adler D, Doering M, Klein PJ, Lennartz KJ (1976) Investigations on pharmakologic induction of partial synchronization of tumor cell proliferation: Its revevance for cytostatic therapy. Cancer treat Rep 60:1959–1979
Klein HO, Gross R (1978) Die Bedeutung der partiellen Synchronisation und der Zellkinetik für den zeitgemäßen Einsatz von Zytostatika. Dtsch Ärztebl 39:2195–2202
Klein HO (1980) Cell kinetic alterations in normal and neoplastic cell populations in vitro and in vivo following vincristine. Cell Tissue Kinet 13:425–434
Lengsfeld AM, Schultze B, Maurer W (1981) Time-lapse studies on the effect of vincristine on Hela cells. Eur J Cancer 17:307–319
Maidhof R, Jellinghaus W, Schultze B, Maurer W (1975) Experimentelle und theoretische Untersuchungen zur Erzeugung einer teilsynchron proliferierenden Zellpopulation mit Vincristin in vivo. Dtsch Med Wschr 100:54–56
Pouillart P, Mathé G, Schwarzenberg L (1973) Essai de recrutement cellulaire par synchronisation partielle pour l'établissement d'une combinaison chimiothérepique. Bull Cancer (Paris) 60:187–196
Pouillart P, Hoang Thy Huong T, Lhéritier J (1974) La leucémmie L 1210, modèle expérimental pour la potentialisation des drogues applicables aux leucémies aigües. Bull Cancer 61:509–526
Schiffer LM, Braunschweiger PG, Poulakos L (1976) Rapid Methods for utilizing cell kinetics for treatment in the C3H/He spontaneous mammary tumor: Effects of vincristine. Cancer Treat Rep 60:1913–1924
Schultze B, Maurer W, Hagenbusch H (1976) A two emulsion autoradiographic technique and the discrimination of the three different types of labelling after double labelling with 3H-and 14C-thymidine. Cell Tissue Kinet 9:245–255
Zeller MJ, Ivankovic S, Schmähl D (1975) Chemotherapy of the transplantable acute leukemia L 5222 in rats. Cancer Res 35:1168–1174
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Schultze, B., Jellinghaus, W., Basler, U. et al. Studies on the so-called “synchronization therapy” of tumors with vincristine. J Cancer Res Clin Oncol 105, 266–274 (1983). https://doi.org/10.1007/BF00395756
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DOI: https://doi.org/10.1007/BF00395756