Summary
Auxotrophic mutants of Saccharomyces cerevisiae and Candida utilis were hybridized through protoplast fusion. Spontaneous, UV- and FPA-induced mitotic segregation indicated that after cell fusion, exclusion of the S. cerevisiae nucleus or nuclear fusion followed by preferential loss of S. cerevisiae chromosomes can take place. Some of the hybrids were stable. One of them, expressed mating and sporulation functions of the S. cerevisiae parent. Thus, markers from both parents could be recovered as mitotic and meiotic segregants.
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References
Brook MR de van, Sierra M, Figueroa LC de (1983) Curr Microbiol 8:13–15
Delgado J, Herrera LS (1981) Acta Microbiol Acad Sci Hung 28:339–345
Delgado J, Herrera LS, Perez C, Lopez R (1979) Can J Microbiol 25:486–490
Ferenczy L, Farkas GL (1980) Advance in protoplast research. Pergamon Press, Oxford
Ferenczy L, Maraz A (1977) Nature 268:524–525
Flores da Cunha M (1970) Genet Res Cambr 16:127–144
Fournier P, Provost A, Bourguignon C, Heslot H (1977) Arch Microbiol 115:142–149
Fried HM, Fink GR (1978) Proc Natl Acad Sci USA 75:4224–4228
Galeotti CL, Clark-Walker GD (1983) Change of gene expression in fusion products between Saccharomyces cerevisiae and Kluyveromyces lactis. In: Manipulation and expression of genes in eukaryotes. Academic Press, Australia
Galzy P, Slonimski PP (1957) Comptes Rend Acad Sci Paris 245:2423
Goodey AR, Bevan EA (1983) Curr Genet 7:69–72
Groves DP, Oliver SG (1984) Curr Genet 8:49–55
Luckermann G, Sipiczki M, Wolf K (1979) Mol Gen Genet 177:185–187
Mortimer RK, Hawthorne DC (1969) Yeast genetics. In: Rose AH, Harrison JS (eds) The yeast, vol 1. Academic Press London, p 385
Mortimer RK, Schild D (1981) Genetic mapp of Saccharomyces cerevisiae. In: Strathern JW, Jones BW, Broach JR (eds) The molecular biology of the yeast Saccharomyces: life cycle and inheritance. Cold Spring Harbor Laboratory, p 641
Nurse PP, Thuriaux P, Nasmyth K (1976) Mol Gen Genet 146:167–178
Perez C, Herrera LS (1981) Exp Mycol 5:15–22
Provost A, Bourguignon C, Fournier P, Ribet AM, Heslot H (1978) FEMS Microbiol letters 31:309–312
Sarachek A, Rhoads DD, Schwarzhoff RH (1981) Arch Microbiol 129:1–8
Sipiczki M (1979) Curr Microbiol 3:37–40
Sipiczki M, Ferenczy L (1977a) Mol Gen Genet 151:77–81
Sipiczki M, Ferenczy L (1977b) FEMS Microbiol letters 2:203–205
Solingen P van, Plaat J van der (1977) J Bact 130:946–947
Spencer JFT, Spencer DM, Whiiiington-Vaughan P, Müller R (1983) Curr Genet 7:159–164
Stahl U (1978) Mol Gen Genet 160:111–113
Vallin C, Ferenczy L (1978) Acta Microbiol Acad Sci Hung 25:209
Wickner RD (1981) Killer systems in Saccharomyces cerevisiae. In: Strathern JW, Jones BW, Broach JR (eds) The molecular biology of the yeast Saccharomyces: life cycle and inheritance. Cold Spring Harbor Laboratory, p 415
Wilson JJ, Khachatourian S, Ingledew WM (1982) Mol Gen Genet 180:95–100
Whittaker PA, Leach SM (1978) FEMS Microbiol letters 4:31–34
Yamamoto M, Fukui S (1977) Agr Biol Chem 41:1829–1830
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Perez, C., Vallin, C. & Benitez, J. Hybridization of Saccharomyces cerevisiae with Candida utilis through protoplast fusion. Curr Genet 8, 575–580 (1984). https://doi.org/10.1007/BF00395702
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DOI: https://doi.org/10.1007/BF00395702