Abstract
We have examined the context of mouse T-cell receptor gamma (Tcr γ) chain variable (V γ) and constant (Cγ) genes among a panel of geographically isolated species of mice. Our Southern hybridization survey with Cγ reveals that essentially three Cγ genes are found among mouse species extending phylogenetically from inbred mice through the feral species Mus pahari. However, a V γ DNA probe detects three to nine V γ restriction fragment bands among the same group of mice. These results suggest that certain feral mice such as M. pahari, M. platythrix, and M. shortridgei have amplified numbers of V γ genes. Studies of individual mice from these particular species indicate the highly amplified V γ content is not the result of a catastrophic gene duplication or deletion event. We conclude that certain species of mice maintain increased content of V γ presumably for increased diversity in a Tcell response.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Allison, J. P., McIntyre, B. W., and Bloch, D.: Tumor-specific antigen of murine T -lymphoma defined with monoclonal antibody. J. Immunol. 129: 2293–2300, 1982
Cazenave, P. A., Bonhomme, F., Guenet, J.-L., and Kindt, T. J.: Correlation of CT β phenotype with origins of laboratory mouse strains. Curr. Top. Microbial. Immunol. 127: 300–306, 1986
Hartman, A. B., D'Hoostelaere, L. A., Potter, M., and Rudikoff, S.: The X-24 VH gene family in inbred mouse strains and wild mice. Curr. Top. Microbial. Immunol. 127: 157–166, 1986,
Haskins, K., Kubo, R., White, J., Pigeon, M., Kappler, J., and Marrack, P.: The major histocompatibility complex-restricted antigen receptor on T cells. I. Isolation with a monoclonal antibody. J. Exp. Med. 157: 1149–1169, 1983
Hayday, A. L., Saito, H., Gillies, S. D., Kranz, D. M., Tanigawa, G., Eisen, H. N., and Tonegawa, S.: Structure, organization, somatic rearrangement of T cell gamma genes. Cell 40: 259–269, 1985
Hedrick, S. M., Cohen, D. I., Nielsen, E. A., and Davis, M. M.: Isolation of cDNA clones encoding T-cell specific membrane-associated proteins. Nature 308: 149–153, 1984
Heilig, J. S., Glimcher, L. H., Kranz, D. M., Clayton, L. K., Greinstein, J. L., Saito, H., Makam, A. M., Burakoff, S. J., Eisen, H. N., and Tonegawa, S.: Expression of the T cell specific γ gene is unnecessary in T cells recognizing class II MHC determinants. Nature 317: 68–70, 1985
Huppi, K., Jouvin-Marche, E., Scott, C., Potter, M., and Weigert, M.: Genetic polymorphism at the κ chain locus in mice: Comparisons of restriction enzyme hybridization fragments of variable and constant region genes. Immunogenetics 21: 445–457, 1985
Huppi, K., D'Hoostelaere, L. A., and Jouvin-Marche, E.: The context of T cell receptor β chain genes among wild and inbred mouse species. Curr. Top. Microbial. Immunol. 127: 291–299, 1986
Iwamoto, A. I., Rupp, F., Ohashi, P. S., Walker, C. L., Pincher, H., Joho, R., Hengartner, H., and Mak, T. W.: T cell-specific γ genes in C57BL/10 mice. J. Exp. Med. 163: 1203–1212, 1986
Klein, J. and Figueroa, R: Polymorphism of the mouse H-2 loci. Immunol. Rev. 60: 23–57, 1981
Kranz, D. M., Saito, H., Heller, M., Takagaki, Y., Haas, W., Eisen, H. N., and Tonegawa, S.: Limited diversity of the rearranged T cell γ gene. Nature 313: 752–755, 1985
LeFranc, M.-P., Forster, A., Baer, R., Stinson, M. A., and Rabbitts, T. H.: Diversity and rearrangement of the human T rearranging γ genes: Nine germline variable genes belonging to two subgroups. Cell 45: 237–246, 1986
Meuer, S. C., Fitzgerald, K. A., Hussey, R. E., Hodgdon, J. C., Schlossman, S. P., and Reinherz, E. L.: Clonotypic structures involved in antigen-specific human T cell function. Relationship to the T3 molecular complex. J. Exp. Med. 157: 705–719, 1983
Potter, M.: Listing of stocks and strains of mice in the genus Mus derived from the feral state. Curr. Top. Microbial. Immunol. 127: 373–395, 1986
Raulet, D. H., Garman, R. D., Saito, H., and Tonegawa, S.: Developmental regulation of T-cell receptor gene expression. Nature 314: 103–107, 1985
Rogers, M. J., Germain, R. N., Hare, J., Long, E., and Singer, D. S.: Comparison of MHC genes among distantly related members of the genus Mus. J. Immunol. 134: 630–636, 1985
Saito, H., Kranz, D. M., Takagaki, Y., Hayday, A. C., Eisen, H. N., and Tonegawa, S.: A third rearranged and expressed gene in a clone of cytotoxic T lymphocytes. Nature 312: 36–40, 1984
Schimke, R. T., Sherwood, S. W., Hill, A. B., and Johnston, R. M.: Over-replication and recombination of DNA in higher eukaryotes: Potential consequences and biological implications. Proc. Nad. Acad. Sci. U.S.A. 83: 2157–2161, 1986
Scott, C. L. and Potter, M.: Polymorphism of C λ genes and units of duplication in the genus Mus. J. Immunol. 132: 2630–2637, 1984a
Scott, C. L. and Potter, M.: Variations in V λ genes in the genus Mus. J. Immunol. 132: 2638–2643, 1984b
Snodgrass, H. R., Dembić, Z., Steinmetz, M., and von Boehmer, H.: Expression of Tcell antigen receptor genes during fetal development in the thymus. Nature 315: 232–233, 1985
Yanagi, Y., Yoshaki, Y., Leggett, K., Clark, S. P., Aleksander, I., and Mak, T. W.: A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains. Nature 308: 145–149, 1984
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Huppi, K., D'Hoostelaere, L., Kiefer, M. et al. The context of T-cell receptor gamma chain genes among wild mouse species. Immunogenetics 24, 304–308 (1986). https://doi.org/10.1007/BF00395535
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00395535