Summary
The in vitro cytotoxic response against H-2 d and H-2 b SV40-transformed fibroblasts was studied in a 40-h 3H-proline assay. A very low response against SV40 TASA is associated with the H-2d antigens on target cells: however, SV40-transformed H-2d cells are as immunogenic as SV40-transformed H-2b cells and prime against H-2b target cells. The data concerning in vitro amplification of the anti-SV40 TASA response and the involvement of cyclophosphamide-sensitive suppressor populations confirm the comparable immunogenicity of SV40-transformed H-2d and H-2b cells and cannot account for the haplotype-related behavior observed with SV40-transformed target cells. The study of the response against allogeneic SV40-transformed cells shows the reverse situation: the lower cytotoxic response is now associated with the H-2b antigens on SV40-transformed cells. As suggested by the data presented here, an interaction between SV40 TASA and H-2 antigens might be postulated.
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Abbreviations
- B6:
-
C57BL/6 mice
- B6D2F1 :
-
(C57BL/6xDBA/2)F hybrid mice
- CB6F1 :
-
(BALB/cxC57BL/6)F1 hybrid mice; E
- T:
-
effector-to-target-cell ratio
- FCS:
-
fetal calf serum
- PBS:
-
phosphate-buffered saline
- TASA:
-
tumor-associated specific antigens
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This work was supported by grant no. 81.01423.96 of Progetto Finalizzato “Controllo della Crescita Neoplastica” from C.N.R., Italy. C.D.G. and G.N. are in receipt of a training grant from C.N.R., Italy; P.-L.L. is in receipt of a fellowship from Fondazione Anna Villa Rusconi, Varese, Italy
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De Giovanni, C., Grilli, S., Lollini, PL. et al. Inverse relationship between anti-SV40 TASA and anti-H-2 cytotoxic responses. J Cancer Res Clin Oncol 106, 117–122 (1983). https://doi.org/10.1007/BF00395389
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DOI: https://doi.org/10.1007/BF00395389