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On the cellular localization of phosphoenolpyruvate carboxylase in Sorghum leaves

Planta volume 151, pages 226–231 (1981)Cite this article

Abstract

The localization of phosphoenol pyruvate carboxylase (EC 4.1.1.3.1.) in the leaf cells of Sorghum vulgare was investigated by using three techniques: the conventional aqueous and non aqueous methods gave conflicting results; the immunocytochemical techniques clearly showed that the enzyme is predominantly located in the cytoplasm of mesophyll cells.

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Abbreviations

PEP:

phosphoenol pyruvate

PAG:

polyacrylamide gel

NADP MDH:

NADP malate dehydrogenase

FITC:

fluorescein isothiocyanate

SAB:

serum albumine bovine

DTT:

dithiothreitol

MDH:

malate dehydrogenase

ME:

malic enzyme

PBS:

phosphate buffer saline

PAP:

peroxidase anti-peroxidase

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Author notes

  1. Arlette Burlet

    Present address: Laboratoire d'Histologie A, Facultés de Médecine, B.P. 184, F-54500, Vandoeuvre les Nancy, France

Affiliations

  1. Laboratoire de Biologie Végétale, Université de Nancy I, ERA au CNRS No 799, C/O No 140, F-54037, Nancy Cedex, France

    Catherine Perrot, Jean Vidal, Arlette Burlet & Pierre Gadal

Authors
  1. Catherine Perrot
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  2. Jean Vidal
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  3. Arlette Burlet
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  4. Pierre Gadal
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Perrot, C., Vidal, J., Burlet, A. et al. On the cellular localization of phosphoenolpyruvate carboxylase in Sorghum leaves. Planta 151, 226–231 (1981). https://doi.org/10.1007/BF00395173

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  • DOI: https://doi.org/10.1007/BF00395173

Key words

  • Immunocytochemistry
  • (PEP carboxylase)
  • PEP carboxylase
  • Sorghum