Abstract
Light-scattering, which can be taken as an indicator of the transthylakoid proton-gradient, and chlorophyll a fluorescence, have been followed simultaneously during re-illumination of spinach leaves at different energy fluence rates and carbon dioxide concentrations. The slow fluorescence transient (“M peak”), which has been associated with photosynthetic induction, was observed in air only at the lower fluence rates used. Data are presented that indicate that M peaks in chlorophyll fluorescence kinetics can only be observed if there is also a simultaneous transient in light-scattering and that these transients are observed when the dark period is relatively long, fluence rate relatively low, and CO2 concentration relatively high.
The results are discussed in relation to the varying demands on ATP by carbon assimilation during induction of photosynthesis at different carbon dioxide concentrations and the manner in which these variations influence the quenching of chlorophyll a fluorescence.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- Chl:
-
chlorophyll
References
Bannister, T.T., Rice, G. (1968) Parallel time courses of O2 evolution and chlorophyll a fluorescence. Biochim. Biophys. Acta 162, 555–580
Cerovic, Z., Sivak, M.N., Walker, D.A. (1984) Slow secondary fluorescence kinetics associated with the onset of photosynthetic carbon assimilation in intact isolated chloroplasts, Proc. R. Soc. London Ser. B 220, 327–338
Dietz, K.-J., Neimanis, S., Heber, U. (1984) Rate limiting factors in leaf photosynthesis. II. Electron transport and ribulose-1,5-diphosphate regeneration. Biochim. Biophys. Acta, in press
Edwards, G.E., Walker, D.A. (1983) C3, C4: Mechanisms and cellular and environmental regulation of photosynthesis, pp. 1–542, Blackwell, Oxford
Heber, U. (1969) Conformational changes of chloroplasts induced by illumination of leaves in vivo. Biochim. Biophys. Acta 180, 302–319
Heber, U., Santarius, K.A. (1965) Compartmentation and reduction of pyridine nucleotides in relation to photosynthesis. Biochim. Biophys. Acta 108, 390–408
Heber, U., Egneus, H., Hanck, U., Jensen, M., Köster, S. (1978) Regulation of photosynthetic electron transport and phosphorylation in intact chloroplasts and leaves of Spinacia oleracea L. Planta 143, 41–49
Kobayashi, Y., Köster, S., Heber, U. (1982) Light scattering, chlorophyll a fluorescence and state of the adenylate system in illuminated spinach leaves. Biochim. Biophys. Acta 682, 44–54
Köster, S., Heber, U. (1982) Light-scattering and quenching of 9-aminoacridine fluorescence as indicators of the phosphorylation state of the adenylate system in intact spinach chloroplasts. Biochim. Biophys. Acta 680, 88–94
Lavorel, J., Etienne, A.-L. (1977) In vivo chlorophyll fluorescence. In: Primary processes of photosynthesis, pp. 203–268, Barber, J., ed. Elsevier/North-Holland Biomedical Press, Amsterdam
Papageorgiou, G., Govindjee (1968) Light induced changes in the fluorescence yield of chlorophyll a fluorescence in vivo. II. Chlorella pyrenoidosa. Biophys. J. 8, 1316–1328
Lilley, R. McC., Chon, C.J., Mosbach, A., Heldt, H.W. (1977) The distribution of metabolites between spinach chloroplasts and medium during photosynthesis in vitro. Biochim. Biophys. Acta 460, 259–272
McAlister, E.C., Myers, J. (1940) The time course of photosynthesis and fluorescence observed simultaneously. Smithson. Misc. Collect. 99, 1–37
Schreiber, U., Groberman, L., Vidaver, W. (1975) Portable, solidstate fluorometer for the measurement of chlorophyll fluorescence induction in plants. Rev. Sci. Inst. 46, 538–542
Sivak, M.N., Walker, D.A. (1983) Some effects of CO2 concentration and decreased O2 concentration on induction fluorescence in leaves. Proc. R. Soc. London Ser. B 217, 377–392
Sivak, M.N., Prinsley, R.T., Walker, D.A. (1983) Some effects of changes in the gas phase on the steady-state chlorophyll a fluorescence exhibited by illuminated leaves. Proc. R. Soc. London Ser. B 217, 393–404
Sivak, M.N., Dietz, K.-J., Heber, U., Walker, D.A. (1985) The relationship between light-scattering and chlorophyll a fluorescence during oscillations in photosynthetic carbon assimilation. Arch. Biochem. Biophys. (in press)
Walker, D.A. (1981) Secondary fluorescence kinetics of spinach leaves in relation to the onset of photosynthetic carbon assimilation. Planta 153, 273–278
Walker, D.A., Sivak, M.N. (1983) Possible relationships between photosynthetic carbon assimilation and chlorophyll fluorescence. In: Proc. Int. Symp. on Kinetics of Photosynthetic Carbon Metabolism in C3 Plants, Tallin, Estonia, USSR, August 1983 (in press)
Walker, D.A., Sivak, M.N., Prinsley, R.T., Cheesbrough, J.K. (1983) Simultaneous measurement of oscillations in oxygen evolution and chlorophyll a fluorescence in leaf pieces. Plant Physiol. 73, 542–549
Witt, H.T. (1979) Energy conversion in the functional membrane of photosynthesis. Analysis by light pulse and electrical pulse methods. The central role of the electric field. Biochim. Biophys. Acta 505, 335–427
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sivak, M.N., Heber, U. & Walker, D.A. Chlorophyll a fluorescence and light-scattering kinetics displayed by leaves during induction of photosynthesis. Planta 163, 419–423 (1985). https://doi.org/10.1007/BF00395152
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00395152