Abstract
Floral induction in seedlings of Pharbitis nil Choisy cv. Violet, with one cotyledon removed, was manipulated by applying various photoperiodic treatments to the remaining cotyledon. Populations of polyadenylated RNA from treated cotyledons were examined to identify messages specifically involved in floral induction. The RNA was translated in vitro using a wheat-germ system, and the resulting translation products were analysed by two-dimensional polyacrylamide gel electrophoresis. Substantial qualitative and quantitative differences were found between mRNA from cotyledons of seedlings kept in continuous light (non-induced) and of seedlings given a 16-h dark period (induced). In contrast, inhibition of flowering with a night-break resulted only in one detectable, quantitative difference in mRNA.
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Abbreviations
- CL:
-
continuous light
- kDa:
-
kilodalton
- NB:
-
16 h darkness+10 min red-light break, 8 h into the dark period
- poly(A)+ RNA:
-
polyadenylated RNA (isolated by binding to a cellulose oligodeoxythymidine affinity column)
- SD:
-
short day (16 h dark)
- SDP:
-
short-day plant
- SDS:
-
sodium dodecyl sulfate
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Lay-Yee, M., Sachs, R.M. & Reid, M.S. Changes in cotyledon mRNA during floral induction of Pharbitis nil CV. Violet. Planta 171, 104–109 (1987). https://doi.org/10.1007/BF00395073
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DOI: https://doi.org/10.1007/BF00395073