Abstract
Ribulose-1,5-bisphosphate carboxylase/oxygelase (RuBPCase) was studied in melon leaves infected by Colletotrichum lagenarium, a fungal pathogen of melons. Electrophoretic analysis of melon leaf proteins indicated a strong effect of infection on RuBPCase, the subunits of which gradually disappeared during the different stages of infection. Enzyme activity also declined 4 d after inoculation and its content, measured by immunoelectrophoresis, decreased to a similar extent. Synthesis of the large and small subunits of RuBPCase was followed by in-vivo pulse-labeling experiments. A drastic decrease in the rate of RuBPCase-subunit synthesis occurred 3 d after inoculation and preceded the appearance of disease symptoms. There was an apparent coordination of the synthesis of the two subunits under these conditions.
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Abbreviations
- LS (SS):
-
Large (small) subunit of RuBPCase
- RuBPCase:
-
ribulose-1,5-bisphosphate carboxylase/oxygenase
- SDS-PAGE:
-
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
- TCA:
-
trichloroacetic acid
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Ranty, B., Roby, D., Cavalié, G. et al. Ribulose-1,5-bisphosphate carboxylase/oxygenase expression in melon plants infected with Colletotrichum lagenarium . Planta 170, 386–391 (1987). https://doi.org/10.1007/BF00395031
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DOI: https://doi.org/10.1007/BF00395031