Abstract
After protoplast fusion somatic hybrid calli were obtained by complementation selection between an albino mutant of Datura innoxia and the wildtype of Atropa belladonna (Krumbiegel and Schieder, 1979. Planta 145, 371–375). In the present study experiments are described concerning leaf and shoot induction on several media supplemented with different combinations and concentrations of hormones. Except for fleshy leaves and embryos, no well-formed shoot could be obtained. However, under standard culture conditions after one and a half years, one line produced numerous green shoots, showing a reduced number of chromosomes from Atropa belladonna. The loss of some chromosomes decreased the degree of somatic incompatibility. The additional appearance of shoots with albino sectors, of total albino shoots, and of green shoots showing a different phenotype, demonstrated that the elimination of the chromosomes occurred not only once, but several times. At least one shoot nearly stable in chromosome content and green subline could be obtained possessing only 6 chromosomes of Atropa belladonna and the original chromosome number of Datura innoxia. Experiments were carried out to test the feasibility of producing sexual hybrids through in vivo and in vitro methods by cross pollination. However, no embryos, seeds, or plantlets were obtained, thus demonstrating that protoplast fusion is the only possibility for obtaining hybrids between these two species.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BAP:
-
6-benzylaminopurine
- 2,4-D:
-
2,4-dichlor-phenoxyacetic acid
- IAA:
-
indoleacetic acid
- NAA:
-
α-naphtaleneacetic acid
- SDS:
-
sodiumdodecylsulfate
References
Binding, H., Nehls, R. (1978) Somatic cell hybridization of Vicia faba+Petunia hybrida. Mol. Gen. Genet. 164, 137–143
Dudits, D., Hadlaczky, G., Bajzár, G.Y., Koncz, C., Lázár, G., Horváth, G. (1979) Plant regeneration from intergeneric cell hybrids. Plant Sci. Lett. 15, 101–112
Dudits, D., Fejér, O., Hadlaczky, G., Koncz, C., Lázár, G., Horváth G. (1980) Intergeneric gen transfer mediated by plant protoplast fusion. Mol. Gen. Genet. 179, 283–288
Gamborg, O.L., Miller, R.A., Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res 50, 151–158
Gerlach, D. (1977) Botanische Mikrotechnik. Eine Einführung. Stuttgart, Thieme-Verlag
Gleba, Y.Y., Hoffmann, F. (1979) Hybrid cell lines Arabidopsis thaliana+Brassica campestris: No evidence for specific chromosome elimination. Mol. Gen. Genet. 165, 257–264
Gleba, Y.Y., Hoffmann, F. (1980) “Arabidobrassica” Plant genome engeneering by protoplast fusion. Naturwissenschaften 66, 547–554
Kao, K.N. (1977) Chromosomal behaviour insomatic hybrids soybean-Nicotiana glauca. Mol. Gen. Genet. 150, 225–230
Krumbiegel, G., Schieder, O. (1979) Selection of somatic hybrids after fusion of protoplasts from Datura innoxia Mill and Atropa belladoma L. Planta 145, 371–375
Monnier, M. (1976) Culture in vitro de l'embryon de Capsella bursa pastoris Moensch. Rev. Cyt. Biol. Vég 39, 1–120
Murashige, F., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497
Power, J.B., Frearson, E.M., Hayward, C., Cocking, E.C. (1975) Some consequences of the fusion and selective culture of Petunia and Parthenocissus protoplasts Plant. Sci. Lett. 5, 197–207
Power, J.B., Berry, S.F., Chupman, J.V., Sink, K.C., Cocking, E.C. (1979) Somatic hybrids between unilateral cross-incompatible Petunia species. Theor. Appl. Genet. 55, 97–99
Rangaswamy, N.S. (1977) Application of in vitro pollination and in vitro fertilization. In: Plant cell, tissue and organ culture pp. 412–425 Reinert, J., Bajaj, Y.P.S., eds., Springer Verlag, Berlin Heildelberg New York
Rietsema, J., Blondel, B. (1959) Growth processes in the embryo and seed. In: Blakeslee: The genus Datura. pp. 196–219 Avery, A.G., Satina, S., Rietsema, J., eds., The Ronald Press Company, New York
Schieder, O. (1980) Somatic hybrids between a herbaceous and two tree Datura species. Z. Pflanzenphysiol. 98, 119–127
Schieder, O., Vasil, I. (1980) Fusion and somatic hybridization. In: Recent advances in plant cell and tissue culture Int. Rev. Cytol., Supplement 11B, pp. 21–46, Vasil, I., ed., Academic Press, New York
Shen, E.Y.E. (1969) Two-solution iron hematoxylin staining (Heidenhain) with HCL differentiation and Tris-buffer neutralization (Notes on technic) Stain Technol. 44, 209–210
Sink, K.C., Power, J.B., Natarella, N.J. (1978) The interspecific hybrid Petunia parodii x Petunia inflata and its relevance to somatic hybridization in the genus Petunia. Theor. Appl. Genet. 53, 205–208
Smith, H.H. (1962) Plant genetic tumors. Proc. Exp. Tumor Res. 15, 138–164
Zenkteler, M. (1980) Intraovarien and in vitro pollination. In: Recent advances in plant cell and tissue culture Internat. Rev. Cytol., Supplement 11B, pp. 137–156, Vasil, I., ed., Academic Press, New York
Zenkteler, M., Melchers, G. (1978) In vitro hybridization by sexual methods and by fusion of somatic protoplasts. Theor. Appl. Genet. 52, 81–90
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Krumbiegel, G., Schieder, O. Comparison of somatic and sexual incompatibility between Datura innoxia and Atropa belladonna . Planta 153, 466–470 (1981). https://doi.org/10.1007/BF00394988
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00394988