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Effect of bleeding site on coagulation results in the Charles river outbred albino SD rat

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Abstract

The UK Home Office currently advises that the amount of blood to be taken during a toxicology study is limited to 15% of an animal's blood volume (equivalent to approximately 1 ml per 100 g of the animal's bodyweight) in a 28 day period. At SmithKline Beecham, blood samples for routine haemtology and clinical chemistry analyses during a study have been obtained from the orbital sinus (OS). Adequate samples can be obtained for all routine assays with the exception of coagulation tests, and the possibility has been investigated of taking terminal coagulation samples from the posterior vena cava (PVC) as part of the post-mortem procedures, and compared the prothrombin (PT) and activated partial thromboplastin (APTT) times obtained on blood samples from each source. There was no biologically significant difference between samples taken from OS and PVC.

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Authors and Affiliations

  1. SmithKline Beecham Pharmaceuticals, Welwyn, Hertfordshire, UK

    C. J. Edwards & J. Fuller

Authors
  1. C. J. Edwards
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  2. J. Fuller
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Additional information

Originally presented at ECCP 93.

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Edwards, C.J., Fuller, J. Effect of bleeding site on coagulation results in the Charles river outbred albino SD rat. Comparative Haematology International 3, 40–42 (1993). https://doi.org/10.1007/BF00394926

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  • DOI: https://doi.org/10.1007/BF00394926

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