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Comparison between maize root cells and their respective regenerating protoplasts: wall polysaccharides

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Abstract

The cell-wall polysaccharides from different parts of maize roots have been analysed. The arabinose, galactose and mannose contents are influenced by cell differentiation, whereas xylose, rhamnose and uronic-acid contents are not. In cap cells, the pectin content is low but rhamnose and fucose are present in larger quantities. The cell-wall polysaccharides from cells of the elongation zone and their respective regenerating protoplasts were also analysed. The walls of the protoplasts contained higher xylose and mannose levels and a much lower level of cellulose than the cells from which they were derived.

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References

  • Asamizu, T., Nishi, A. (1979) Biosynthesis of cell wall polysaccharides in cultured carrot cells. Planta 146, 49–54

    Google Scholar 

  • Asamizu, T., Nishi, A. (1980) Regenerated cell wall of carrot protoplasts isolated from suspension cells. Physiol. Plant. 48, 207–212

    Google Scholar 

  • Benbadis, A. (1964) Analyse de la croissance et de la multiplication de cellules isolées in vitro. Rev. Cytol. Biol. Vég. 27, 225–237

    Google Scholar 

  • Blaschek, W., Franz, G. (1983) Influence of growth conditions on the composition of cell wall polysaccharides from cultured tobacco cells. Plant Cell Reports 2, 257–260

    Article  Google Scholar 

  • Blaschek, W., Haass, D., Koehler, H., Franz, G. (1981a) Cell wall regeneration by Nicotiana tabacum protoplasts: chemical and biochemical aspects. Plant. Sci. Lett. 22, 47–57

    Article  Google Scholar 

  • Blaschek, W., Haass, D., Koehler, H., Franz, G. (1981b) Cell wall regeneration by Nicotiana tabacum protoplasts: Chemical and biochemical Aspects. In: Cell walls 81, pp. 109–118, Robinson, D.G., Quader, H. eds. Wissenschaftliche Verlagsgesellschaft mbH, Stuttgart

    Google Scholar 

  • Blaschek, W., Koehler, H., Semler, U., Franz, G. (1982) Molecular weight distribution of cellulose in primary cell walls. Planta 154, 500–555

    Google Scholar 

  • Blumenkrantz, N., Asboe-Hansen, G. (1973) New method for quantitative determination of uronic acids. Anal. Biochem. 54, 484–489

    PubMed  Google Scholar 

  • Boffey, S.A., Northcote, D.H. (1975) Pectin synthesis during cell regeneration of plasmolyzed tobacco leaf cells. Biochem. J. 150, 433–440

    PubMed  Google Scholar 

  • Bowles, D.J., Northcote, D.H. (1976) The size and distribution of polysaccharides during their synthesis within the membrane system of maize root cells. Planta 128, 101–106

    Google Scholar 

  • Cocking, E.C. (1972) Plant Cell protoplasts-isolation and development. Annu. Rev. Plant Physiol. 23, 29–50

    Article  Google Scholar 

  • Dever, J.E., Bandurski, R.S., Kivilaan, A. (1968) Partial chemical characterization of corn root cell walls. Plant Physiol. 43, 50–56

    Google Scholar 

  • Franz, G. (1972) Polysaccharid-metabolismus in den Zellwänden wachsender Keimlinge von Phaseolus aureus. Planta 102, 334–347

    Google Scholar 

  • Galbraith, D.W. (1981) Microfluorimetric quantification of cellulose biosynthesis by plant protoplasts using Calcofluor white. Physiol. Plant. 53, 111–116

    Google Scholar 

  • Galbraith, D.W., Shields, B.A. (1982) The effects of inhibitors of cell wall synthesis on tobacco protoplast development. Physiol. Plant. 55, 25–30

    Google Scholar 

  • Hanke, D.E., Northcote, D.H. (1974) Cell wall formation by soybean callus protoplasts. J. Cell. Sci. 14, 29–50

    PubMed  Google Scholar 

  • Harris, P.J., Northcote, D.H. (1970) Pattern of polysaccharide biosynthesis in differentiating cells of maize root tips. Biochem. J. 120, 479–491

    PubMed  Google Scholar 

  • Heller, R. (1953) Recherches sur la nutrition minérale des tissus végétaux cultivés in vitro. Ann. Sci. Nat. Bot. Biol. Veg. 14, 1–223

    Google Scholar 

  • Herth, W., Meyer, Y. (1977) Ultrastructural and chemical analysis of the wall fibrils synthesized by tobacco mesophyll protoplasts. Biol. Cellulaire 30, 33–40

    Google Scholar 

  • Horine, R.K., Ruesink, A.W. (1972) Cell wall regeneration around protoplasts isolated from Convolvulus tissue culture. Plant Physiol. 50, 438–445

    Google Scholar 

  • Kato, K., Noguchi, M. (1976) Sugar composition of cell wall polysaccharides of suspension cultured tobacco cells. Agric. Biol. Chem. 40, 1923–1928

    Google Scholar 

  • Klein, A.S., Montezinos, D., Delmer, D.P. (1981) Cellulose and 1,3-glucan synthesis during the early stages of wall regeneration in soybean protoplasts. Planta 152, 105–114

    Google Scholar 

  • Masuda, Y., Pilet, P.E. (1983) Mechanical properties and polysaccharide nature of the cell walls of maize root. Physiol. Plant. 59, 512–517

    Google Scholar 

  • Pilet, P.E. (1973) Effect of cell wall degrading enzymes on protoplasts transaminase activity. Experientia 29, 29–50

    Google Scholar 

  • Pilet, P.E. (1977) Growth inhibitors in growing and geostimulated maize roots. In: Plant growth regulation, pp. 115–128, Pilet, P.E., ed., Springer, Berlin Heidelberg New York

    Google Scholar 

  • Pilet, P.E. (1979) Kinetic of the light-induced georeactivity of maize roots. Planta 145, 403–404

    Google Scholar 

  • Pilet, P.E. (1980) Hormonal control of root georeaction: some light effects. In: Plant growth substances, pp. 450–461, Skoog, F., ed. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Pilet, P.E., Prat, R., Roland, J.C. (1972) Morphology, RNase and transaminase of root protoplasts. Plant Cell Physiol. 13, 297–309

    Google Scholar 

  • Pilet, P.E., Roland, J.C. (1971) Effects of abscisic acid on the growth and ultrastructure of tissues cultivated in vitro. Cytobiologie 4, 41–61

    Google Scholar 

  • Prat, R., Roland, J.C. (1971) Etude ultrastructural des premiers stades de néoformations d'une enveloppe par les protoplastes végétaux séparés mécaniquement de leur paroi. C.R. Acad. Sci. Ser. D 273, 165–168

    Google Scholar 

  • Saeman, J.F., Buhl, J.L., Harris, E.F. (1945) Quantitative Saccharification of wood and cellulose. Ind. Eng. Chem. Anal. Ed. 17, 35–37

    Google Scholar 

  • Senn, A., Pilet, P.E. (1980) Isolation and some physiological properties of maize root protoplasts. Z. Pflanzenphysiol. 100, 299–310

    Google Scholar 

  • Senn, A., Pilet, P.E. (1981) Electrophoretic mobility, zeta potential and surface changes of maize root protoplasts. Z. Pflanzenphysiol. 102, 19–32

    Google Scholar 

  • Senn, A., Pilet, P.E. (1982) Indoleacetic acid and abscisic acid effects on the electrokinetic properties of maize root protoplasts. In: Plasmalemma and tonoplast: their functions in the plant cell, pp. 69–76, Marmè, D., Marrè, E., Hertel, R., eds. Elsevier Biomedical Press, Amsterdam New York Oxford

    Google Scholar 

  • Takeuchi, Y., Komamine, A. (1978) Composition of the cell wall formed by protoplasts isolated from cell suspension cultures of Vinca rosea. Planta 140, 227–232

    Google Scholar 

  • Takeuchi, Y., Komamine, A. (1980) Regenerated cell wall of carrot protoplasts isolated from suspension-cultured cells. Physiol. Plant. 48, 207–212

    Google Scholar 

  • Willison, J.H.M. (1976) Synthesis of cell walls by higher plant protoplasts. In: Microbial and plant protoplasts, pp. 283–298, Peberdy, J.F., ed. Academic Press, New York, London

    Google Scholar 

  • Willison, J.H.M., Klein, A.S. (1982) Cell wall regeneration by protoplasts from higher plants. In: Cellulose and other natural polymer systems; biogenesis, structure and degradation, pp. 61–85, Brown, R.M., jr., ed. Plenum Press, New York, London

    Google Scholar 

  • Wright, H. (1975) Polysaccharides of root cap slime from five maize varieties. Phytochemistry 14, 759–763

    Article  Google Scholar 

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Pilet, PE., Blaschek, W., Senn, A. et al. Comparison between maize root cells and their respective regenerating protoplasts: wall polysaccharides. Planta 161, 465–469 (1984). https://doi.org/10.1007/BF00394579

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  • DOI: https://doi.org/10.1007/BF00394579

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